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pGEMHE:mTREK-1(A286F)

Plasmid

#224746

Purpose

In vitro transcription for Oocyte expression of mTREK-1(A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

A286F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-S131C)

Plasmid

#224759

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S562C

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/G171F)

Plasmid

#224749

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / G171F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-wt)

Plasmid

#224758

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/A286F)

Plasmid

#224748

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / A286F

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-MYC-As-NF-kB3

Plasmid

#180205

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Use

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-412

Promoter

CMV

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-HNRNPK-ts1

Plasmid

#174250

Purpose

HNRNPK knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HNRNPK shRNA (HNRNPK Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-HNRNPK-ts2

Plasmid

#174251

Purpose

HNRNPK knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HNRNPK shRNA (HNRNPK Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Article

Sun et al J Am Coll Cardiol Basic Trans Science. n

Insert

Cre

Use

AAV

Tags

Expression

Mutation

Promoter

cTnT

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG011_Spike_Parental_D614G_3xFlag

Plasmid

#191571

Purpose

SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G mutation. Last 19 amino acids truncated.

Promoter

CMV

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SOX17

Plasmid

#222925

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOX17

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOX17 (SOX17 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

Plasmid

#170827

Purpose

PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d ANXA2 gRNA2

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mVprBP pc2953

Plasmid

#201698

Purpose

Expresses of GFP-tagged VprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pmcherry-mVprBP pc2954

Plasmid

#201699

Purpose

Expresses of mcherry-tagged mVprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

Plasmid

#220494

Purpose

To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting LIG4 exon 3 (LIG4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB2

Plasmid

#180201

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Tags

N terminal Myc tag

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pC1_oROS-HT

Plasmid

#216413

Purpose

Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-FKBPflex-ELL-CAAX

Plasmid

#220076

Purpose

an extra long linker attached to a CAAX domain with an mCherry tag

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

CAAX (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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