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Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-KRAB_C-term
Plasmid#136889PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codonDepositorInsertKRAB repressor domain
UseLentiviralTagsNoneExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (L)
Plasmid#170624PurposeFor the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539EDepositorInsertLyn11-myc-LOV2GIVe (L)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (D)
Plasmid#170625PurposeFor the mammalian expression of mLOV2GIVe dark-mimicking mutation C450SDepositorInsertLyn11-myc-LOV2GIVe (D)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH198_pE12_hyCal2-eGFP
Plasmid#160411PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal2::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH200_pE12_hyCal7-eGFP
Plasmid#160413PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal7::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH201_pE12_hyCal7-mCherry
Plasmid#160414PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC7) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal7::mCherry
UseOtherTagsmCherryExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH202_pE12_hyCal9-eGFP
Plasmid#160415PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to eGFPDepositorInsertpDONR221 HyCal9::eGFP
UseOtherTagseGFPExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH203_pE12_hyCal9-mCherry
Plasmid#160416PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC9) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal9::mCherry
UseOtherTagsmCherryExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc460
Plasmid#161940PurposeCarries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoterDepositorInsertpSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
UseTagsExpressionBacterialMutationPromoterPPhlFAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc120
Plasmid#161950PurposeContains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmidDepositorInsertpSB4A3-PECF20-B30-mScarlet-I-B15
UseTagsExpressionBacterialMutationPromoterPECF20Available sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc393
Plasmid#161953PurposeDemonstrates chemically induced dimmerization of acVHH. T7 RNAP polymerase reporter plasmidDepositorInsertpSB4A3-T-PT7-B30-mScarlet-I-3T
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc330
Plasmid#161952PurposeContains elements for building reporter plasmid for IBM. SrpR reporter plasmidDepositorInsertpSEVA171-PSrpR-B30-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPSrpRAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc050
Plasmid#161938PurposeCarries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoterDepositorInsertpSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
UseTagsExpressionBacterialMutationPromoterPlux2Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc259
Plasmid#161951PurposeContains elements for building reporter plasmid for IBM.TetR reporter plasmidDepositorInsertpSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter
UseTagsExpressionBacterialMutationPromoterPTet*Available sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2365 - Intron GG1 - 250bp no PATC
Plasmid#159883PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertIntron GG1 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2345 - Intron GG2 - 250bp no PATC
Plasmid#159884PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertIntron GG2 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2366 - intron GG3 - 250bp no PATC
Plasmid#159885PurposeGolden Gate compatible intron with no PATCs for negative controlsDepositorInsertintron GG3 - 250bp no PATC
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
Plasmid#159888PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-1 (w ATG)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only