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myc-Med22 pBacPAK8

Plasmid

#15374

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med22 (MED22 Human)

Use

Baculovirus construction

Tags

myc

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med31 pBacPAK8

Plasmid

#15375

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med31 (Med31 Mouse)

Use

Baculovirus construction

Tags

myc

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
myc-Med19 pBacPAK8

Plasmid

#15376

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med19 (Med19 Mouse)

Use

Baculovirus construction

Tags

myc

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

Plasmid

#205631

Purpose

Expression of TnpBmax in mammalian cells

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB optimized for mammalian expression

Use

Tags

NLS-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-mmPCSK9

Plasmid

#205634

Purpose

reRNA guide targeting Mus Musculus PCSK9

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmPCSK9 (Pcsk9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1:mTRESK(S116C)

Plasmid

#224772

Purpose

In vitro transcription for Oocyte expression of mTRESK(S116C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 18 (Kcnk18 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S116C

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C/Q258K)

Plasmid

#224764

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S118C/Q258K

Promoter

T7

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1

Plasmid

#224773

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1(S131C)

Plasmid

#224774

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C)

Plasmid

#224744

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A)

Plasmid

#224751

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131A)

Plasmid

#224745

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S156C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(G171F)

Plasmid

#224747

Purpose

In vitro transcription for Oocyte expression of mTREK-1(G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

G171F

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/K271Q)

Plasmid

#224750

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / K271Q

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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