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Plasmid#188898PurposeHuman lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertdCas9
UseLentiviralTagsHA-2xNLS and P2A-GFPExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTY152 EGFP-T2A-ORF6 SARS-CoV-1
Plasmid#204974PurposeCo-express EGFP and SARS-CoV-1 ORF6DepositorInsertOpen Reading Frame 6 (ORF6 SARS-CoV-1)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CycK-GFP (1-270AA) in Cilantro (Codon optimized)
Plasmid#169930PurposeOverexpression of 1-270AA CycK-GFP fusionDepositorInsertCCNK (CCNK Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
Plasmid#65443PurposeExpression of EPHB3 and GFP in mammalian cellsDepositorInsertEPHB3 (EPHB3 Human)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA
Plasmid#108871PurposeExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafishDepositorInserts5xU6:sgRNA
Cas9-t2A-GFP
UseCRISPRTagsExpressionMutationPromoterU6 and hsp70Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-scFv GCN4-sfGFP-VP64
Plasmid#122133PurposeExpress a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoterDepositorInsertscFv GCN4-sfGFP-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBa-eGFP-flag-BicD2 594-FKBP
Plasmid#63569PurposeBicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBPDepositorInsertBicaudal D protein (Bicd2 Mouse)
UseTagsFKBP, eGFP, and flagExpressionMammalianMutationno start methionine, aa1-594Promoterchicken Beta ActinAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast
Plasmid#205549PurposeExpress EPN24-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194283PurposeEF1a-dCas9-KRAB-GFP with Slc1a3 sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP-SFPQ-IRES-mCherry
Plasmid#166950PurposeLentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoterDepositorInsertSFPQ (SFPQ Human)
UseLentiviralTagsGFP and mCherryExpressionMutationPromotereF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
Plasmid#132389PurposeExchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauWT 2N4R-EGFP (MAPT Human, Synthetic)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianMutationPromoterTREtightAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)
Plasmid#136627PurposePlasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-LAMA-R59
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH
Plasmid#131508PurposeEndogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)DepositorUseLentiviralTagsExpressionMammalianMutationPromoterhUBCAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
UseTagsExpressionWormMutationPromoterlim-4Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
Plasmid#189795PurposeExpress GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cellsDepositorInsertbeta-globin (HBB Human, Mouse)
UseTagsExpressionMammalianMutationPromoterPonasterone A inducibleAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls
Plasmid#64113PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only