We narrowed to 58,585 results for: %s

1. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET41sIII_GST-ALC1-K77R

   Plasmid

   #174813

   Purpose

   Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-K77R (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K77R

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET41sIII_GST-ALC1-E175Q

   Plasmid

   #174814

   Purpose

   Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-E175Q (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E175Q

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Bmpr2ΔEC gRNA resistant

   Plasmid

   #163413

   Purpose

   Expresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The extracellular domain (27-150 aa) is deleted. …

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Bmpr2ΔTail gRNA resistant

   Plasmid

   #163412

   Purpose

   Expresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The C-terminal tail domain (530-1038 aa) is delet…

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2 Register 1/3 Tnos:YFP:5'UTR (GB1507)

   Plasmid

   #160583

   Purpose

   Part 1/3 for assembly of site-specific recombination based Registers. Composed of the reversed sequences of the TNos terminator,YFP CDS and 5' UTR of 35S promoter (TNos:YFP:5'UTR)

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   YFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. deltaLoop-MIDAS

   Plasmid

   #171605

   Purpose

   Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

   Insert

   MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
   

   Use

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Mutation

   Replaced amino acids 4458-4496 with ASGSGS linker

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XLone-Puro Cas13d-eGFP U6 RUNX1 g1

    Plasmid

    #155185

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. XLone-Puro Cas13d-eGFP U6 RUNX1 g2

    Plasmid

    #155186

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d RUNX1 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-Puro Cas13d-eGFP U6 SOX17 g1

    Plasmid

    #155187

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA1

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA1
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-EGFP-DmTral-R42EF44A_C

    Plasmid

    #146061

    Purpose

    Insect Expression of DmTral-R42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42EF44A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    R42E, F44A. Additionally, two silent mutations A1…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer10-UBC9-sh3

    Plasmid

    #168987

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-EGFP-DmTral-R42E_B

    Plasmid

    #145988

    Purpose

    Insect Expression of DmTral-R42E

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42E (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent mutations A198C, A387G and a mutation …

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDECKO_mCherry_KLF4

    Plasmid

    #140104

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO_mCherry_NFE2

    Plasmid

    #140105

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDECKO_mCherry_TREML2

    Plasmid

    #140106

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge