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pVL47 - pSCR>>

Plasmid

#116013

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSCR:GR-LhG4:tSCR:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA-hNEUROG2-iRPT

Plasmid

#140764

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with TRE-hNEUROG2

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2
NEUROG2 (NEUROG2 Human)

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A2-C106R

Plasmid

#31123

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2, mutation C106R. Wild type seq…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCAFNF-PSDd1.2-GFP

Plasmid

#125581

Purpose

Flp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

PSDd1.2-GFP (Dlg4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE2

Plasmid

#160766

Purpose

Suppress POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaRING-LIN-41

Plasmid

#52718

Purpose

retroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 12-91

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

T301N/N323S

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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LbCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126643

Purpose

Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1

Plasmid

#45822

Depositor

Joseph Opferman

Article

Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

Insert

AS9(Mito MTS)-Delta N67-mMCL1

Expression

Mammalian

Mutation

ATP Synthase subunit 9 is used as a Mito Targetin…

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only
GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLD-hygro-EnVM

Plasmid

#24590

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VM

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pX330A-Yap1-E1-#1

Plasmid

#171517

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E2-#1

Plasmid

#171518

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

Plasmid

#120296

Purpose

AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

Rosa26 gRNA (SpCas9 scaffold)

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGMC00034 (aka pMC0235)

Plasmid

#210751

Purpose

Non-targeting guide RNA

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

Non-targeting guide RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSW554 - pOp5:SP-mVenus-HDEL

Plasmid

#116006

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pet23a_RBPMS_FL

Plasmid

#59392

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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