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Plasmid#76967Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3C3DepositorInsertPIK3C3 (PIK3C3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPKm-231
Plasmid#90500PurposepSIN - EF-1alpha - MTS - tFd - P2A - MTS - tFNR, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd) and Ferredoxin-NADP(+) oxireductase (FNR), under EF-1alpha promoterDepositorInsertmito-tFD and mito-tFNR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-232
Plasmid#90501PurposepSIN - EF-1alpha - MTS tHO1 - P2A - MTS - tPCYA, vector encoding for mitochondrial-tagged Thermosynechococcus elongates Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase, under EF-1alpha promoterDepositorInsertmito-tHO1 and mito-tPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-234
Plasmid#90507PurposepSIN - EF-1alpha - MTS sHO1 - P2A - MTS - sPCYA, vector encoding for mitochondrial-tagged Synechococcus sp. Heme Oxigenase-1 (HO1) and Phycocyanobilin:ferredoxin oxireductase (PcyA), under EF-1alpha promoterDepositorInsertmito-sHO1, mito-sPCYA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED30
Plasmid#15431DepositorInsertMED30 (Med30 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE MD-deltaN-ER
Plasmid#13496DepositorInsertMyoD1-Estrogen Receptor Fusion Protein (Myod1 Mouse, Human)
UseRetroviralTagsExpressionMammalianMutationMyoD (containing a deletion in aa3-56) was fused …PromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_3x_siRNA
Plasmid#59893Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has three siRNA-like targets (fully c…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_RBPMS
Plasmid#65745Purposeexpresses RFP-tagged RBPMS in mammalian cellsDepositorInsertRBPMS (RBPMS Human)
UseTagsRFPExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
RIOK3 gRNA (BRDN0001147200)
Plasmid#76539Purpose3rd generation lentiviral gRNA plasmid targeting human RIOK3DepositorInsertRIOK3 (RIOK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TNIK gRNA (BRDN0001149184)
Plasmid#75852Purpose3rd generation lentiviral gRNA plasmid targeting human TNIKDepositorInsertTNIK (TNIK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SGK1 gRNA (BRDN0001146486)
Plasmid#77459Purpose3rd generation lentiviral gRNA plasmid targeting human SGK1DepositorInsertSGK1 (SGK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PIK3C3 gRNA (BRDN0001147879)
Plasmid#76966Purpose3rd generation lentiviral gRNA plasmid targeting human PIK3C3DepositorInsertPIK3C3 (PIK3C3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNUTN6His Y426F/Y517F hTFNG
Plasmid#70084PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobeDepositorInsertmutated human serum transferrin (TF Human)
UseTagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Ty426Phe, Tyr517PhePromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS2
Plasmid#59390PurposeDestination vector with RBPMS2 for stable cell line generationDepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Ani)
Plasmid#45577PurposeCodes for the SSA-TLR with the target site for I-Ani I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Ani I TS
+3 mCherry
3' iRFP arm
UseLentiviralTagsExpressionBacterial and MammalianMutationembedded I-Ani I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED31
Plasmid#15437DepositorInsertMED31 (Med31 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4-delta-kinase-PY3 mutant
Plasmid#17802DepositorInsertErbB4 (ERBB4 Human)
UseTagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment, amino acids 988-1292, …PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Med18 pBacPAK8
Plasmid#15371DepositorInsertMed18 (MED18 Human)
UseBaculovirus constructionTagsFLAG and HisExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med11 pBacPAK8
Plasmid#15373DepositorInsertMed11 (Med11 Mouse)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only