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pBABE (hygro) mEOS2 hYAP 1-2delta

Plasmid

#124162

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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SGK1 gRNA (BRDN0001147869)

Plasmid

#77458

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SGK1 (SGK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3F-CTD

Plasmid

#105052

Purpose

Overexpression of APOBEC3F C-terminal domain in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3F (A3F) C-terminal domain (APOBEC3F Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

it contains only C terminal domain of A3F

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 K321A/R325A

Plasmid

#89287

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Lys-321 and Ar…

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-GEI-17(133-509)

Plasmid

#87056

Purpose

Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)

Use

Tags

6xHis-TEV and GAGGGS linker

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_TAP_FAM86A

Plasmid

#85116

Purpose

mammalian expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Use

Tags

CBP-SBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A S974A

Plasmid

#69814

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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gh116

Plasmid

#106786

Purpose

expression of gRNA targeting GCNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT2 (GCNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pPN060

Plasmid

#91593

Purpose

Express sgRNA targeting human CYP26B1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CYP26B1 (CYP26B1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN065

Plasmid

#91598

Purpose

Express sgRNA targeting human DGKI

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DGKI (DGKI Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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SEPHS2 gRNA (BRDN0001147864)

Plasmid

#77663

Purpose

3rd generation lentiviral gRNA plasmid targeting human SEPHS2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SEPHS2 (SEPHS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV-hCas9 R4-R3

Plasmid

#62132

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

human codon optimized Cas9

Use

CRISPR; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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SNRK gRNA (BRDN0001146665)

Plasmid

#76733

Purpose

3rd generation lentiviral gRNA plasmid targeting human SNRK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SNRK (SNRK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestGFP_RBPMS

Plasmid

#65744

Purpose

expresses GFP-tagged RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET32b-MED19

Plasmid

#15438

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Use

Tags

His and Trx

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

Plasmid

#60393

Purpose

Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Synthetic, Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
p-miR-Report + MT miR-181 BS in NLK 3UTR

Plasmid

#22791

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only