-
-
pDONR223-LOC390877
Plasmid#23373DepositorInsertLOC390877 (LOC390877 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CFLAR_WT_V5
Plasmid#82936PurposeGateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCFLAR (CFLAR Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ICOSLG-Fc(DAPA)-AviTag-6xHis
Plasmid#156690PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertICOSLG (ICOSLG Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-CAMK1G
Plasmid#23813DepositorInsertCAMK1G (CAMK1G Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CASP8_WT_V5
Plasmid#82999PurposeGateway Donor vector containing CASP8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCASP8 (CASP8 Human)
UseGateway entry vectorTagsExpressionMutationAA 184_191delERSSSLEGinsGEELCGVMTISD; D194R; 196_…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-NES-ZapCV2
Plasmid#222950PurposeGenetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorInsertNES-ZapCV2 (NES Synthetic)
UseTagsECFP (Enhanced Cyan Fluorescent Protein), Nuclear…ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo2.1 K+ channel [N11/33R-2b]
Plasmid#206651PurposeMammalian Expression Plasmid of anti-Slo2.1 K+ channel (Mouse). Derived from hybridoma N11/33-2b.DepositorInsertanti-Slo2.1 K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnt2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ASCC3_GRIK2
Plasmid#205774PurposeExpress mEGFP-tagged fusion protein, ASCC3_GRIK2 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N SM
Plasmid#37964DepositorInsertSM (SM, spliced BSLF2+BMLF1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA
Plasmid#184549PurposeRetroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tagDepositorUseRetroviralTags3xFLAG and HA tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_NUP214:1-2090
Plasmid#211294PurposeMammalian expression of full-length human NUP214. C-terminal HiBiT tag.DepositorInsertNUP214:M1-S2090 (NUP214 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationP574S natural variant (VAR_045691). S1872N annot…PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_NUP214:1-2090
Plasmid#211220PurposeMammalian expression of full-length human NUP214. N-terminal HiBiT tag.DepositorInsertNUP214:M1-S2090 (NUP214 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationP574S natural variant (VAR_045691). S1872N annot…PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_CFAP52:1-620
Plasmid#211235PurposeMammalian expression of full-length human CFAP52. N-terminal HiBiT tag.DepositorInsertCFAP52:M1-S620 (WDR16 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationA91V C199S E336K annotated sequence conflictsPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_CFAP52:1-620
Plasmid#211309PurposeMammalian expression of full-length human CFAP52. C-terminal HiBiT tag.DepositorInsertCFAP52:M1-S620 (WDR16 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationA91V C199S E336K annotated sequence conflictsPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PML:1-781
Plasmid#211090PurposeMammalian expression of full-length human PML isoform 13. C-terminal NanoLuc luciferase tag.DepositorInsertPML:M1-L781 (PML Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 13 . E224D annotated sequence conflict. …PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DCAF13:1-445
Plasmid#211273PurposeMammalian expression of full-length human DCAF13. C-terminal HiBiT tag.DepositorInsertDCAF13:M1-K445 (DCAF13 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationA86S annotated sequence conflictPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only
Holiday Schedule: Addgene will be closing at 1:00 pm EST on November 27th and remain closed through the 29th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about estimated ship dates, please feel free to track your order status or contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
How are we doing? Share feedback for a chance to win $50
Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more
Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more
