Addgene

We narrowed to 13,552 results for: pET

Showing: 11861 - 11880 of 13552 results

Results per page:

Sort results by:

pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Use

Tags

histag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Use

Tags

G protein and His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pECFP-IETD2x-Venus

Plasmid

#24536

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-IETD2x-Venus

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pN-PITCh-HBTH

Plasmid

#127883

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-HBTH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAF-MutL

Plasmid

#86607

Purpose

E. coli MutL expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG Enthoprotin Full Length

Plasmid

#47419

Depositor

Peter McPherson

Article

Wasiak et al J Cell Biol. 2002 Sep 2;158(5):855-62. d

Insert

Enthoprotin

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pN-PITCh-H3FAID

Plasmid

#127884

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F-AID

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pN-PITCh-HF

Plasmid

#127882

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

GFP-Puro-H3F

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p6895 PHAGE-P CMVT N-HA-hE6AP I wt

Plasmid

#37601

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I wt (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV tet regulatable

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
BRPF1 (3L42)

Plasmid

#25339

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

BRPF1_P1 (BRPF1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBN280

Plasmid

#86731

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Ptph-1::FLP D5

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-pcDronpa2

Plasmid

#78184

Purpose

Expresses pcDronpa2 in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa2

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
CBX4

Plasmid

#25237

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CBX4_02 (CBX4 Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCAG-HA-rat Nrxn2beta AS4(-)

Plasmid

#58268

Purpose

Expression plasmid for HA-tagged rat Neurexin 2beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn2beta AS4(-) (Nrxn2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge