Addgene

Showing: 11861 - 11880 of 21160 results

Results per page:

Sort results by:

pAct:dCas9-GCN4

Plasmid

#78899

Purpose

Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-10XGCN4

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

David Root, John Doench

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
eSpCas9(1.1)-Puro

Plasmid

#117686

Purpose

The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

Depositor

Luc Leyns

Article

Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

Insert

eSpCas9(1.1)

Use

CRISPR

Tags

PuroR (R166H)

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

U6

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ePPE

Plasmid

#183095

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpCas9

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9 nickase for Split-PE (pJUL2497)

Plasmid

#190104

Purpose

Expresses nickase SpCas9(H840A) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

nSpCas9

Use

Tags

XTEN-bpNLS and bpNLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS157 CRISPEY RT/Cas9

Plasmid

#114454

Purpose

Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

Depositor

Hunter Fraser

Article

Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

Insert

SpCas9, Ec86-RT

Use

CRISPR

Tags

SV40-NLS

Expression

Yeast

Mutation

S. cerevisiae codon optimized

Promoter

GAL1-GAL10

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgLenti

Plasmid

#105996

Purpose

single sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.3-efSaCas9-BlastR

Plasmid

#220971

Purpose

Expresses human codon-optimized efSaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

efSaCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

Plasmid

#117687

Purpose

U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

Depositor

Erik Sontheimer

Article

Gao et al Methods Enzymol. 2019;616:365-383. doi:

Inserts

Kanamycin cassette
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_CnCas12f1

Plasmid

#204996

Purpose

CnCas12f1 heterologous expression plasmid

Depositor

Quanjiang Ji

Article

Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

Insert

CnCas12f1

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ePPEplus

Plasmid

#205241

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Tags

Expression

Plant

Mutation

Detailed in manuscript

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ePPE-spG

Plasmid

#183096

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nspGCas9-NC-nls-MLV-Nls

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A in SpGCas9

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Expression

Mutation

Promoter

hybrid constitutive promoter 35SPPDK

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-hAsCpf1(TATV) (pY220)

Plasmid

#89353

Purpose

Expresses humanized AsCpf1 variant that recognizes TATV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge