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Showing: 11861 - 11880 of 20673 results

  2. pCMV-PE2-P2A-PuroR

    Plasmid
    #196962
    Purpose
    Expresses PE2 with puromycin resistance
    Depositor
    Leopold Parts
    Insert
    PE2-P2A-Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    196962-plasmid-map-sequence-id-440213

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  3. pCFD3-dUG-DH1

    Plasmid
    #138963
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    DH1 oligo
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138963-plasmid-map-sequence-id-274780

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  7. ipUSEPR-sg-Hs-ACE2-A2

    Plasmid
    #188687
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188687-plasmid-map-sequence-id-375123

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  8. ipUSEPR-sg-Hs-ACE2-A4

    Plasmid
    #188688
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188688-plasmid-map-sequence-id-375122

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  9. ipUSEPR-sg-Hs-TMPRSS2-A5

    Plasmid
    #188689
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgTMPRSS2 (TMPRSS2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188689-plasmid-map-sequence-id-375120

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  10. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  11. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  12. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  13. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

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  14. pKLV-gRNA-BTRC-MGMT_2

    Plasmid
    #136416
    Purpose
    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136416-plasmid-map-sequence-id-271757

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  15. pCSD_SpCas9MT3-FKBP_SL

    Plasmid
    #107302
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107302-plasmid-map-sequence-id-204632

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  16. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid
    #107306
    Purpose
    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    TALE_VEGFA-TS3
    Use
    CRISPR
    Tags
    3x Flag, 2xNLS and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107306-plasmid-map-sequence-id-204787

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  17. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid
    #87150
    Purpose
    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87150-plasmid-map-sequence-id-174939

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  20. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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Showing: 11861 - 11880 of 20673 results