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Plasmid#176244PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Entry IL36RN S113L
Plasmid#58321Purposeentry vector for human IL36RN S113L mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationS113L: c.338C>T, p.Ser113LeuPromoterCMVAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF366
Plasmid#88019PurposeDonor Vector containing ZNF366 transcription factor, part of the Human TFome CollectionDepositorInsertZNF366 (ZNF366 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41s-GST-ATF6a-AD
Plasmid#64774Purposebacterial expression of GST-tagged ATF6 (1-150)DepositorInsertATF6a-AD (1-150) (ATF6 Human)
UseTagsGSTExpressionMammalianMutationC-term deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
J7AAV-HDR Fah.2
Plasmid#73451PurposeJ7AAV-HDR U6sgFah.2 template2 to repair Fah mutationDepositorInsertFah (Fah Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
IGFALS-bio-His
Plasmid#52180PurposeExpresses full-length Insulin-like growth factor-binding protein complex ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertIGFALS (IGFALS Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)4
Plasmid#127522PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF175
Plasmid#88551PurposeDonor Vector containing ZNF175 transcription factor, part of the Human TFome CollectionDepositorInsertZNF175 (ZNF175 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR
Plasmid#118622Purposeexpression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neuronsDepositorInsertcdc42E7-E6 3'UTR (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationchanged ttgcttt to cggtaag (496 - 502 nt in 3…Promotersynapsin I (rat)Available sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Jak3-SH2
Plasmid#46449DepositorInsertJak3 (JAK3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 366-494PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cherry-SRBC
Plasmid#69492PurposeExpression of fluorescently tagged SRBC in mammalian cellsDepositorInsertSRBC (Cavin3 Mouse)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP
Plasmid#75121PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRG1PromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP
Plasmid#75119PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomainDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the bromodomain of BRD1PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25-VWA
Plasmid#64772PurposeMammalian expression of Flag-tagged Med25-VWADepositorInsertMed25-VWA (MED25 Human)
UseTagsFlagExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
J8AAV-HDR Ctnnb1.1
Plasmid#73452PurposeJ8AAV-HDR U6sgCtnnb1.1 templateDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEE
Plasmid#159746PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1430
Plasmid#29225PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle170 (POGZ Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-Entry IL36RN R102W
Plasmid#58323Purposeentry vector for human IL36RN R102W mutantDepositorInsertIL36RN (IL36RN Human)
UseEntry vectorTagsmyc-DDKExpressionMutationR102W: c.304C>T, p.Arg102TrpPromoterCMVAvailable sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb10-PIR-SH2
Plasmid#46436DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 355-548PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only