Addgene

We narrowed to 34,864 results for: lat

Showing: 11841 - 11860 of 34864 results

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pcDNA3-eNOS-GFP

Plasmid

#22444

Depositor

William Sessa

Article

Sowa et al J Biol Chem. 1999 Aug 6. 274(32):22524-3

Insert

WT eNOS-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Puro

Plasmid

#124302

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pZLCv2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106357

Purpose

Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG and HA

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Article

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. aureus scaffold CARGO array

Plasmid

#191317

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMEH280 NLS dTomato 3x high affinity MS2 loops

Plasmid

#168211

Purpose

Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 RagA

Plasmid

#73031

Purpose

Expresses human RagA from a CMV promoter for stable expression in mammalian cells

Depositor

David Sabatini

Article

Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

Insert

rragA (RRAGA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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JD639

Plasmid

#160398

Purpose

Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

35S::vitis miR396-resistant GRF4-GIF1 chimera

Use

Binary vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/ES-EnvA-VSVg-WPRE-pA

Plasmid

#87221

Purpose

to package wild type EnvA psudotyped lentivirus

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

EnvA-VSVg

Use

Mouse Targeting and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS-SV40-EGFP

Plasmid

#46919

Purpose

Target EGFP gene that is stably integrated into HEK293 genome

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

GFP

Use

CRISPR and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-HaloTagx2

Plasmid

#177113

Purpose

Expresses Vps8-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

VPS8 (VPS8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 2xdBroccoli

Plasmid

#169233

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-2xdBroccoli

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight

Plasmid

#205077

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Article

Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

Insert

T7RNAP Cyslight (T7p07 Bacteriophage T7)

Use

Tags

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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