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Plasmid#218233PurposeHDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae geneDepositorInsertputative metabolite transport protein
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-dUG-D01
Plasmid#138962PurposeUsed in the editing of Drosophila kkvDepositorArticleInsertD01 oligo
UseCRISPR; To synthesize grnaTagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-KIF11
Plasmid#188685Purposecontrol sgRNADepositorInsertsgKIF11 (KIF11 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-CDK1
Plasmid#188684Purposecontrol sgRNADepositorInsertsgCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CUP1-1
Plasmid#166086PurposePlasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertCUP1-1 (CUP1-1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH3
Plasmid#166084PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.DepositorInsertPromoter of TDH3 (TDH3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)
Plasmid#160631PurposeMultiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.DepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterU626Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2-udhA
Plasmid#65819PurposegltA-udhA targeting guide RNA array E. coli , Low Phosphate InductionDepositorInsertgltA2-udhA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-TREG3G-PE2-rtTA3G-P2A-eGFP
Plasmid#196971PurposePiggybac vector with doxycyclin-inducible prime editor for mammalian cellsDepositorInsertsCas9-RT
rtTA3G-P2A-eGFP
UseTagsExpressionMammalianMutationPromoterPGK and TREG3GAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV238-vPE
Plasmid#225258PurposeMammalian expression of vPEDepositorInsertvPE
UseCRISPRTagsExpressionMammalianMutationR221K K848A H982A N1317RPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
Plasmid#140082PurposeEntry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoterDepositorInsertpT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
UseIn vitro transcription (mrna synthesis)TagsExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-TMPRSS2-A6
Plasmid#188690PurposesgRNADepositorInsertsgTMPRSS2 (TMPRSS2 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-AAVS1-sg
Plasmid#194721Purposepx458 with guide RNA that target hAAVS1DepositorArticleInsertCas9 (AAVS1 Streptococcus pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINO4-NLS-Pfv-Sapphire-HO
Plasmid#203660PurposeYeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.DepositorArticleInsertPfV
UseCRISPRTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAIO-Ef1a-PE2-GFP:KCNQ2-C201R
Plasmid#185060PurposeEf1a driven PE2 plasmid with pegRNA for editing C201R mutation in KCNQ2 gene. See Addgene plasmid #184445DepositorInsertU6:pegRNA:scaffold:PBS+RT template
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-BCL2L1-A1
Plasmid#188692PurposesgRNADepositorInsertsgBCL2L1 (BCL2L1 Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PasCas13b-gRNA-EGFP(W58stop)
Plasmid#180507PurposePasCas13b-gRNA targeting EGFP(W58stop) for RNA editingDepositorInsertPasCas13b-gRNA targeting EGFP(W58stop)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PasCas13b-gRNA-Nluc(W12stop)
Plasmid#180506PurposePasCas13b-gRNA targeting Nluc(W12stop) for RNA editingDepositorInsertPasCas13b-gRNA targeting Nluc(W12stop)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:Ble:Cas9
Plasmid#112208PurposeGolden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.DepositorInsertsCas9
SheBle
UseCRISPR; Diatom, phaeodactylumTagsPhaeodactylum fcp promoter and terminator and YFPExpressionMutationPhaeodactylum Fcp promoterPromoterPhaeodactylum FcpAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only