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Plasmid#75153PurposeMembrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.DepositorInsertmVenus-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYT
Plasmid#53300PurposeContains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coliDepositorInsertcrtE, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14_GFP5_Encap
Plasmid#86408PurposeExpresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 5aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)
Plasmid#182370PurposeDendra2-based translation reporterDepositorInsertmtIF3 (Mtif3 Mouse)
UseTagsPal-Dendra2ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO
Plasmid#68405PurposeThe prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoterDepositorInserts3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO
UseCRISPRTagsmTurquoise2 (BFP)ExpressionMammalianMutationPromoterSynthetic Probasin ARRx2 and U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlue-loxPuro-APEX-PML_LASA
Plasmid#140273Purposea targeting plasmid for APEX-PMLDepositorInsertAPEX2
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP) mnuctdTomato
Plasmid#172342PurposemnuctdTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
jk56
Plasmid#87009Purposegenomic Sindbis virus plasmid for expression of SYNseq componentsDepositorInsertsNrx1B
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-myrSNAP-CON
Plasmid#60099PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T5
Plasmid#161492PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pGAP T1
Plasmid#161488PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-LEU2-I-SceI
Plasmid#67595PurposeI-SceI expression regulated by tetracycline inducible system in S. pombeDepositorInsertsnmt terminator
I-SceI
TetR
UseGene targeting in yeastTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMH120
Plasmid#40593PurposeExpresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expressionDepositorUseTagsExpressionYeastMutationPromoterADH1 and TEFAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab5aa
Plasmid#109716PurposeMultisite gateway vector for 3' tagging with rab5aaDepositorInsertrab5aa (rab5aa Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2
Plasmid#128166PurposeMonomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)DepositorInsertmTagBFP2-T2A-sfGFP-iCre-ERT2
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only