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Plasmid#40338DepositorInsertBCL-6Δ (BCL6 Human)
UseTagsExpressionMammalianMutationMissing four of six zinc fingers (see note below)…PromoterCMVAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pORANGE CACNG8-GFP KI #1
Plasmid#131473PurposeEndogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)DepositorInsertgRNA and GFP donor (Cacng8 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)
Plasmid#154838PurposeInsertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selectionDepositorInsertsynthetic guide::∆HYGR::unc-54 3' UTR
UseCRISPR and Cre/LoxTagsExpressionWormMutation∆HYGR is promoterless and encodes aa 60-341PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp8
Plasmid#157695Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp8 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YES1 (T348I) in pLX303
Plasmid#42564DepositorInsertYES1 (YES1 Human)
UseLentiviralTagsExpressionMammalianMutationT348IPromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED18
Plasmid#15433DepositorInsertMED18 (MED18 Human)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS
Plasmid#123188Purposebinary plant vector for transient fLUC (with intron) expressionDepositorInsert2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
49535-sgFMR1_E3B
Plasmid#157783PurposeExpresses a sgRNA targeting the 3rd exon of human FMR1 geneDepositorInsertFMR1 (FMR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_U6_sgRNA_Mettl3C
Plasmid#165420PurposesgRNA construct for targeting Mettl3 C-terminusDepositorInsertPspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
FB029
Plasmid#119713PurposePromoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter paf
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterPromoter pafAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB030
Plasmid#119714PurposeTerminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator paf
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp15
Plasmid#157721Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp15 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p7055 pHAGE-P-CMVt-N-HA-GAW-COPZ1
Plasmid#100145PurposeExpresses COPZ1DepositorInsertCOPZ1 (COPZ1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1alpha
Plasmid#124139PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp14-HF
Plasmid#157719Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoterDepositorInsertNsp14-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-EF1a-V5-Emerin
Plasmid#120246PurposeExpresses V5-tagged Emerin in lentiviral vectorDepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p6589 MSCV-IP N-HAonly CHEK2
Plasmid#34903DepositorInsertCHEK2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmiR-96 cluster promoter
Plasmid#62517PurposemicroRNA 183-96-182 cluster promoter luciferase plasmidDepositorInsertpromoter of microRNA-183-96-182 cluster
UseTagsRenSP (optimized renilla luciferase gene)ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4
Plasmid#112839Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH3 (N-term) domain of Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only