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Plasmid#194231PurposeLentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB1-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
Plasmid#194232PurposeLentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-LCB3-PDGFR TMD-T2A-mCherry
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorInsertEF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro hGSDME gRNA1
Plasmid#223519Purposeknocking out hGSDME in human cellsDepositorInsertGSDME (GSDME Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIN-WP-mPGK-GDNF-IRES-tTA-KRAB
Plasmid#225488PurposeTet-on lentiviral vector for controlled expression of GDNFDepositorInsertTetracycline-controlled transactivator fused with KrĂ¼ppel-associated box (KRAB) domain following an internal ribosome entry site
UseLentiviralTagsExpressionMammalianMutationPromoterMouse phosphoglycerate kinase 1Available sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spGFP
Plasmid#225486PurposeTet-on lentiviral vector for controlled expression of secretable and permeable EGFPDepositorInsertIgκ leader and TAT peptide
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUT-spC3GFP
Plasmid#225487PurposeTet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFPDepositorInsertSecretable and permeable exoenzyme C3 transferase
UseLentiviralTagsEGFPExpressionMammalianMutationAmino acids 30-251PromoterHuman ubiquitin CAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8221 pLIX-PTPN14
Plasmid#221643PurposeDoxycycline-inducible overexpression of human WT PTPN14DepositorInsertPTPN14 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8224 pLIX-PTPN14 ΔPPXY1/2
Plasmid#221644PurposeDoxycycline-inducible overexpression of human PTPN14 ΔPPXY1/2 mutantDepositorInsertPTPN14 ΔPPXY1/2 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationdeletion of the first and second (tandem) PPXY mo…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8552 pLIX-PTPN14 ΔPPXY3/4
Plasmid#221645PurposeDoxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutantDepositorInsertPTPN14 ΔPPXY3/4 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationdeletion of the third and fourth PPXY motifs as d…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2
Plasmid#221646PurposeConstitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutantDepositorInsertPTPN14 ΔPPXY1/2 (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationdeletion of the first and second (tandem) PPXY mo…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4
Plasmid#221647PurposeConstitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutantDepositorInsertPTPN14 ΔPPXY3/4 (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationdeletion of the third and fourth PPXY motifs as d…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8189 pHAGE N-V5 PTPN14 C1121S
Plasmid#221648PurposeConstitutive ectopic expression of human PTPN14 C1121S mutantDepositorInsertPTPN14 C1121S (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationC1121S mutation at the putative catalytic sitePromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8389 LentiCRISPRv2 Neo sgNT-1
Plasmid#221649PurposeExpression of non-targeting control sgRNADepositorInsertnon-targeting sgNT-1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8390 LentiCRISPRv2 Neo sgNT-2
Plasmid#221650PurposeExpression of non-targeting control sgRNADepositorInsertnon-targeting sgNT-2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8391 LentiCRISPRv2 Neo sgPTPN14-1
Plasmid#221651PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-1 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8392 LentiCRISPRv2 Neo sgPTPN14-3
Plasmid#221652PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-3 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)
Plasmid#217798PurposeLentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoterDepositorInsertXTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR and LentiviralTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterEF1aAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-OgNLuc
Plasmid#208851PurposeExpresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.DepositorInsertOgNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Antares
Plasmid#208852PurposeExpresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.DepositorInsertAntares
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only