Addgene

We narrowed to 13,552 results for: pET

Showing: 11781 - 11800 of 13552 results

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p6647 MSCV-P C-Flag HA 2aE7

Plasmid

#35137

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV2a E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
p6649 MSCV-P C-FlagHA 38 E7-Kozak

Plasmid

#35143

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV38 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p6656 MSCV-P C-FlagHA 25E7-Kozak

Plasmid

#35022

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Use

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1(-)

Plasmid

#15260

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 (Nlgn1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Lbpro (29-195)

Plasmid

#110759

Purpose

Bacterial expression of Lbpro

Depositor

Tim Skern

Article

Guarne et al EMBO J. 1998 Dec 15;17(24):7469-79. doi:

Insert

Lb pro

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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HDAC6 (3GV4)

Plasmid

#25297

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HDAC6_42 (HDAC6 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acid residues 1109-1215

Promoter

Available since

Aug. 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits
NW50

Plasmid

#133445

Purpose

Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW50

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL723

Plasmid

#140393

Purpose

Expresses HucR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-HucR-T

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-GFP

Plasmid

#127888

Purpose

Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn3beta AS4(-)

Plasmid

#58269

Purpose

Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn3beta AS4(-) (Nrxn3 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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tUI-HA

Plasmid

#122662

Purpose

Express tUI-HA protein in E. coli

Depositor

Robert Cohen

Article

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tUI-HA

Use

Tags

HA-tag and His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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human p53-(1-360)

Plasmid

#24865

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-360.

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-CaNAR2

Plasmid

#64728

Purpose

FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Use

Tags

Cerulean3 and YPet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Use

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1beta AS4(+)

Plasmid

#59410

Purpose

Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Nrxn1beta AS4(+) (Nrxn1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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