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pCfB3036(XI-1 MarkerFree)

Plasmid

#73274

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2903(XI-2 MarkerFree)

Plasmid

#73275

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB3039(XII-2 MarkerFree)

Plasmid

#73279

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pCfB2909(XII-5 MarkerFree)

Plasmid

#73281

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-Fbxo7(ΔF-box)-IRES-GFP

Plasmid

#171787

Purpose

retrovirus production

Depositor

Heike Laman

Article

Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

Insert

Fbxo7 isoform 1 (FBXO7 Human)

Use

Retroviral

Tags

Expression

Mutation

deletion of aa335-367

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
QQR1 variant

Plasmid

#138564

Purpose

Expresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

QQR1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

G1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-HOXA9

Plasmid

#131608

Purpose

expression of human HOXA9 in mammalian cells

Depositor

Filipe Pereira, Kateri Moore

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

HOXA9 (HOXA9 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMyc-C1-Cep123 (CCDC123/Cep89)

Plasmid

#69836

Purpose

This plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tag

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-IGF2

Plasmid

#133383

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IGF2 (IGF2 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTargeting_Mettl3C_FKBP-V

Plasmid

#165421

Purpose

Template for knockin FBKP-V into Mettl3 C-terminus

Depositor

Neil Brockdorff

Article

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)

Use

Mouse Targeting

Tags

FKBP-V

Expression

Mutation

Promoter

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SIMx6

Plasmid

#116938

Purpose

Bacterial expression vector for 6 sim

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

6 sim

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-HISx6-SUMOx10

Plasmid

#116937

Purpose

Bacterial expression vector for 10 sumo

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

10 SUMO

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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VRER variant

Plasmid

#138561

Purpose

Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VRER

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pR2Q306c

Plasmid

#101027

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMV_mIFP IRES EGFP

Plasmid

#72443

Purpose

Expressing mIFP and EGFP in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

mIFP T2A HO1 IRES EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only