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Plasmid#194428PurposePlasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)DepositorInsertPyruEth
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_75_Donor_Tag_RPL13A-mTurq2
Plasmid#194415PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRproDepositorInsertmTurquoise2 CDS
UseSynthetic Biology; Used as donor dna in genome in…TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA23-sgRNA vector-U6-sgTS2 (SpCas9)
Plasmid#199454PurposeU6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)DepositorInsertTS2 sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA28-pMa-PspCas13b crRNA-TS1
Plasmid#199459PurposeU6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )DepositorInsertTS1 crRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA17-CRISPR-Tag (11XTS1) for dSpCas9
Plasmid#199448PurposeCRISPR-Tag sequence that can be efficiently bound by dSpCas9DepositorInsertCRISPR-Tag
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)
Plasmid#199460PurposeExpression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryosDepositorInsertZebrafish NarTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPINA7A2mTFP1(F)
Plasmid#193774PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertmTFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPINA7 synthetic promoter carrying binding sites f…Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPANI2 synthetic promoter carrying binding sites f…Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2a
Plasmid#186818PurposeFluorescent reporter for Camk2a expressionDepositorInsertCamk2a promoter (Camk2a Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2a promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS001
Plasmid#194012PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertROSA-TRE-C16V5-G-pA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Ccu-sgRNA-SV40-puro
Plasmid#192130PurposeExpresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCcu-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Hsp1-sgRNA-SV40-puro
Plasmid#192129PurposeExpresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertHsp1-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HySp5-310
Plasmid#192995PurposePlasmid that encodes for Hydra Sp5, lacking the SP boxDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP boxPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB2AR-miRFP718nano
Plasmid#195751PurposeBeta-2-adrenergic receptor internally tagged with miRFP718nanoDepositorInsertB2AR-miRFP718nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMyosin-miRFP718nano
Plasmid#195748PurposeLC-Myosin labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertmiRFP718nano-LC-Myosin
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP718nano
Plasmid#195743PurposeMonomeric near-infrared fluorescent protein miRFP718nano in bacterial plasmidDepositorInsertmiRFP718nano
UseTagsHisExpressionBacterialMutationPromoteraraCAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGas-miRFP718nano
Plasmid#195752PurposeG protein alpha-subunit internally tagged with miRFP718nanoDepositorInsertG protein alpha-s-miRFP718nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS002
Plasmid#194013PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex3 XTR f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only