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Showing: 11781 - 11800 of 11907 results

  5. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pAGE2.0

    Plasmid
    #165984
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165984-plasmid-map-sequence-id-375867

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  7. pBGE1.0

    Plasmid
    #165986
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    165986-plasmid-map-sequence-id-375342

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  10. pTT5-hSDC1-CS2

    Plasmid
    #52367
    Purpose
    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R95Q and S194A (according to numbering in Fig. 2A…
    Promoter
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. HCA2-DuET

    Plasmid
    #213308
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213308-plasmid-map-sequence-id-430846

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  15. FSHR-DuET

    Plasmid
    #213242
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FSHR (FSHR Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213242-plasmid-map-sequence-id-430799

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  16. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  17. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    The second base of the first intron of B2M (From …
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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  18. pAGE1.0

    Plasmid
    #165983
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165983-plasmid-map-sequence-id-378731

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  19. pBGE2.0

    Plasmid
    #165987
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165987-plasmid-map-sequence-id-375875

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  20. pAGE3.0

    Plasmid
    #165985
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165985-plasmid-map-sequence-id-375864

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Showing: 11781 - 11800 of 11907 results