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Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2
Plasmid#48146PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1
Plasmid#48145PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 RDF ( GB1498)
Plasmid#160579PurposeStreptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 integrase (GB1496)
Plasmid#160578PurposeStreptomyces phage PhiC31 integrase, complete CDSDepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 PhiC31 integrase (GB1561)
Plasmid#160586PurposeStreptomyces phage PhiC31 integrase, adapted for C-terminal fusionsDepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTARA
Plasmid#31491DepositorInsertRNA polymerase
UseTagsExpressionBacterialMutationN823DPromoterAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRVV210
Plasmid#87628PurposeDrives Bxb1 expression in the D. melanogaster germ line.DepositorInsertBxb1
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFPT7
Plasmid#28012DepositorInsertT7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_Omega2_Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1678)
Plasmid#160642PurposeModule for the dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.DepositorInsertGRLacIBDGal4AD / RDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)
Plasmid#160617PurposeTU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)
Plasmid#160614PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)
Plasmid#160615PurposeTU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromotermini35sAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHD-DsRed-attP-w+
Plasmid#80898PurposeVector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.DepositorInsertsattP
LoxP-3xP3-DsRed-LoxP
GMR-white
UseHigh copy, amp resistanceTagsExpressionMutationPromoter3xP3 and GMR::hsp70Available sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pML1342
Plasmid#32376DepositorInsertint L5; xylEm; ttsbiA, ttsbiB
UseBacterial integration vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
p15aTetT7mut-fuseYFP
Plasmid#28013DepositorInsertEYFP-fuse, T7 RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only