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Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneUseTags6xhisExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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UMG-SP-3_pET29b
Plasmid#215435PurposeExpression construct for UMG-SP-3 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
ThcCut2 _pET29b
Plasmid#215430PurposeExpression construct for ThcCut2 gene, codon optimized for expression in E. coliDepositorInsertThcCut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MsmegA _pET29b
Plasmid#215411PurposeExpression construct for MsmegA gene, codon optimized for expression in E. coliDepositorInsertMsmegA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB-SCY_pET29b
Plasmid#215415PurposeExpression construct for NylB-SCY gene, codon optimized for expression in E. coliDepositorInsertNylB-SCY
UseTagsExpressionBacterialMutationR187S/F264C/D370YPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB'_pET21b
Plasmid#215416PurposeExpression construct for NylB' gene, codon optimized for expression in E. coliDepositorInsertNylB'
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CdRdhA
Plasmid#217824PurposeExpresses N-His-CdRdhA in E. coliDepositorInsertN-His-CdRhdA
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DhFld
Plasmid#217821PurposeExpresses N-His-DhFld in E. coliDepositorInsertDhFld
UseTagsHis6xExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA
Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-YPet
Plasmid#215747PurposeExpresses MS2 coat protein (MCP) fused to YPetDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PunyEEER
Plasmid#208322PurposeBacterial expression of the His-tagged peptide PunyEEER.DepositorInsertPunyERRE
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET50_G495R∆2CARD
Plasmid#216805PurposeExpresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domainsDepositorInsertmelanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
UseTagsExpressionBacterialMutationG495R/∆1-287PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET50_G495R MDA5
Plasmid#216803PurposeExpresses a single point mutant of melanoma differentiation-associated protein 5DepositorInsertmelanoma differentiation-associated protein 5 (IFIH1 Human)
UseTagsExpressionBacterialMutationG495RPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16_Caspase-4_p10
Plasmid#214308PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only