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Plasmid#133562PurposeSwap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry
Plasmid#170185PurposeExpression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.DepositorInsertsEc86 RT
Retron Ec86 msr-msd
BFP-to-GFP conversion donor template An
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCBh and hU6Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralTagsExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…PromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1/mCherry-DMDEx23
Plasmid#211367PurposeExpresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)DepositorInsertmCherry-DMDEx23
UseTagseGFPExpressionMammalianMutationmCherry interrupted by mdx dystrophin exon 23 bet…PromoterCMVAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…TagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-GFP-LoxP-SNAP-TERT
Plasmid#71391PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.DepositorInsertTERT (TERT Human)
UseHr donorTagsFLAG-SNAPExpressionMutationPromoterEndogenous TERT promotorAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR; Gateway donorTagsHA Tag and VP160ExpressionMutationD10A;H840A nuclease-deficientPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 MLK1
Plasmid#60540PurposeDonor vector for human MLK1DepositorInsertMLK1 (MAP3K9 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-FKBP-CYB5Atail
Plasmid#108141PurposeFor recruitment of ERDepositorUseTagsExpressionMammalianMutationCYB5A(100-134)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-V159N
Plasmid#118383PurposeGateway compatible donor vector with actin-V159N.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationV159NPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1788
Plasmid#154317PurposeCT Donor-cassette for SapTrapDepositorInsertlinker::TEV::BioTag::AID*::3xFLAG donor for CT slot in the SapTrap cloning system
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1787
Plasmid#154316PurposeNT Donor-cassette for SapTrapDepositorInsertlinker::TEV::BioTag::AID*::3xFLAG donor for NT slot in the SapTrap cloning system
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-G168D,Y169D
Plasmid#118384PurposeGateway compatible donor vector with actin-G168D,Y169D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationG168D,Y169DPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro XLone-CCND2
Plasmid#179845Purposedonor plasmid for inducible expression of CCND2 in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188747PurposeDonor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterUbCAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188743PurposeDonor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterRSVAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188744PurposeDonor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188745PurposeDonor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchr21Available sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#188746PurposeDonor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locusDepositorArticleInsertSurface marker and mCitrine reporter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternonTATAchrXAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EhT
Plasmid#215546PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only