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Plasmid#39291DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_2
Plasmid#185017PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1-HLx10
Plasmid#108137PurposeRecruitable SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(1-520):[EAAAR]10:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic
Plasmid#32737DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …Promoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENTR/U6 HDAC4 shRNA
Plasmid#32220DepositorInsertshRNA against murine HDAC4 (Hdac4 Mouse)
UseRNAi; Gateway u6 entry vectorTagsExpressionMutationPromoterhuman U6Available sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
PX458_ARID5B_1
Plasmid#86350PurposeEncodes gRNA for 3' target of human ARID5BDepositorInsertgRNA against ARID5B (ARID5B Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSECB sgMTHFD2L_2
Plasmid#106307PurposeExpress Cas9 and sgRNA targeting MTHFD2LDepositorInsertsgRNA targeting MTHFD2L (MTHFD2L Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPG_1
Plasmid#86344PurposeEncodes gRNA for 3' target of human CEBPGDepositorInsertgRNA against CEBPG (CEBPG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-PRMT9 L182A/D183A/I184A/G185A
Plasmid#79676Purposemammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutantDepositorInsertPRMT9 (PRMT9 Human)
UseTagsEGFPExpressionMammalianMutationL182A/D183A/I184A/G185A catalytic mutantPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S-HLx8
Plasmid#108138PurposeRecruitable inactive SAC1 with extended linkerDepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo U6-SpAi9R-U6-SpDMDR
Plasmid#78609PurposeExpresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23DepositorInsertU6-SpAi9R-U6-SpDMDR
UseUnspecifiedTagsExpressionMutationPromoterU6Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPG_2
Plasmid#86345PurposeEncodes gRNA for 3' target of human CEBPGDepositorInsertgRNA against CEBPG (CEBPG Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgGTTG.CMV/CB-Gluc
Plasmid#121512PurposeExpresses sgRNA targeting the intron in the MADM alleles (GT and TG).DepositorInsertsgGTTG
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PSMD14 E5.4 gRNA
Plasmid#90858Purpose3rd generation lentiviral gRNA plasmid targeting human PSMD14DepositorInsertPSMD14 (Guide Designation E5.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CBX1 A3.5 gRNA
Plasmid#90566Purpose3rd generation lentiviral gRNA plasmid targeting human CBX1DepositorInsertCBX1 (Guide Designation A3.5)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PM BRD7 sh2
Plasmid#41928DepositorInsertBRD7 (BRD7 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
AA244
Plasmid#215945PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDB_131
Plasmid#216095PurposeCas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive controlDepositorInsertCD97, CD4, CD26, CD274 guides
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-v2-ALDH1A3gRNA1
Plasmid#189746PurposeThe construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.DepositorInsertCas9, ALDH1A3 gRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only