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B3-1

Plasmid

#111471

Purpose

translational initiation factor 2

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational initiation factor 2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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B4-5

Plasmid

#111479

Purpose

ribosome recycling factor

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

ribosome recycling factor

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-A114-N40

Plasmid

#50561

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-A114-N40

Use

Cloning vector

Tags

Expression

Mutation

Promoter

lac

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Utf1

Plasmid

#13369

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

undifferentiated embryonic cell transcription factor 1 (Utf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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B1-1

Plasmid

#111463

Purpose

Val-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Val-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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B1-2

Plasmid

#111464

Purpose

Met-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4134388

Plasmid

#152696

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BsaI-tagRFP-BsaI-pUC-kan

Plasmid

#170771

Purpose

test plasmid for Golden Gate assembly, encodes fluorescent red protein

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

tagRFP

Use

Tags

Expression

Mutation

Promoter

none

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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B7-4

Plasmid

#111492

Purpose

Met-tRNA formyltransferase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA formyltransferase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B6-4

Plasmid

#111488

Purpose

Phe-tRNA synthetase A

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Phe-tRNA synthetase A

Use

Tags

6xHis

Expression

Bacterial

Mutation

A81S

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B7-3

Plasmid

#111491

Purpose

Tyr-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Tyr-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B6-1

Plasmid

#111485

Purpose

Phe-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Phe-tRNA synthetase B

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B6-2

Plasmid

#111486

Purpose

Pro-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Pro-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B6-3

Plasmid

#111487

Purpose

Ser-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Ser-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDJSVT89

Plasmid

#109303

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Use

Tags

TamA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCB6-ZONAB-A

Plasmid

#136641

Purpose

Expression of ZONAB isoform A in mammalian cells

Depositor

Karl Matter, Maria Balda

Article

Balda et al EMBO J. 2000 May 2;19(9):2024-33. doi: 1

Insert

ZONAB isoform A (YBX3 Canine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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B5-2

Plasmid

#111481

Purpose

Arg-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Arg-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B3-3

Plasmid

#111473

Purpose

Glu-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Glu-tRNA synthetase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDJSVT90

Plasmid

#109304

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Use

Tags

TamA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge