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Plasmid#140451PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin-AflII
Plasmid#106404PurposeEmpty guide RNA cloning vector with an AflII site added from Addgene 41824DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterU6Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC1455-LMNA 5'HA-mCherry-CRISPR-Tag_V2-3'HA (HDR donor)
Plasmid#164042PurposeCRISPR donor plasmid to insert mCherry-harboring CRISPR-TagV2 into the N-terminus of human LMNA geneDepositorInsertmCherry-CRISPR-TagV2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
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RcSxph D794A
Plasmid#194040PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationD794APromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph D794E
Plasmid#194041PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationD794EPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph D794N
Plasmid#194042PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationD794NPromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph Y795A
Plasmid#194043PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationY795APromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph Y558A/I782A
Plasmid#194044PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
UseTagsHis10 and mEGFP,ExpressionInsectMutationY558A/I782APromoterPolyhedrinAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Vibrio_campbellii
Plasmid#224404PurposeFor expression of the type I CBASS system encoded by Vibrio campbelliiDepositorInsertEndonuclease_VcCdnG_AGS-C
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
NL1
Plasmid#218404PurposeNL1 insert in AAV9 capsidDepositorInsertNL1 7mer (LTTEGRR)
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
UseTagsExpressionBacterialMutationS226P/R228SPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VC155
Plasmid#194046PurposeTransient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VN154
Plasmid#194045PurposeTransient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192404PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2252
Plasmid#170404PurposeFor expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 455-479)PromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_FLT1
Plasmid#187784PurposeMAC-tagged gene expression of human FLT1DepositorInsertFLT1 (FLT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_FLT4
Plasmid#187769PurposeMAC-tagged gene expression of human FLT4DepositorInsertFLT4 (FLT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G
Plasmid#146404PurposeBacterial Expression of DmDCP1_328-366-mut2DepositorInsertDmDCP1_328-366-mut2 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only