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Showing: 2321 - 2360 of 7564 results

  4. pOCC114

    Plasmid
    #118904
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with TEV protease, two glycines
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118904-plasmid-map-sequence-id-232331

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  10. pCS2+/C-Halo

    Plasmid
    #184418
    Purpose
    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Article
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    Halo-tag
    Expression
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184418-plasmid-map-sequence-id-373502

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  20. pMpGWB316

    Plasmid
    #68644
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged tdTomato (tdTo…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68644-plasmid-map-sequence-id-121804

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  29. csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

    Plasmid
    #191751
    Purpose
    Microtubule marker together with nuclear envelope marker driven by bidirectional promoter
    Depositor
    Jay C Dunlap
    Article
    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do
    Inserts
    NCU04288 (NCU04288 Neurospora crassa)
    bml (Bml Neurospora crassa)
    Use
    Tags
    Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    191751-plasmid-map-sequence-id-379333

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  30. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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  31. pOCC167

    Plasmid
    #118909
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118909-plasmid-map-sequence-id-232266

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  37. cPE

    Plasmid
    #183549
    Purpose
    Expresses compact Prime Editor in mammalian cells
    Depositor
    Wen Xue
    Article
    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do
    Insert
    SpCas9 nickase (H840A), Reverse Transcriptase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183549-plasmid-map-sequence-id-365389

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  40. p123shy1-mCherry-HA

    Plasmid
    #67875
    Purpose
    encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.
    Depositor
    Armin Djamei
    Article
    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do
    Insert
    shy1
    Use
    Expression in ustilago maydis
    Tags
    HA and mCherry
    Expression
    Mutation
    Promoter
    shy1
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67875-plasmid-map-sequence-id-121040

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Showing: 2321 - 2360 of 7564 results