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Plasmid#123190Purposebinary plant vector for transient tGFP (with intron) and fLUC (with intron) expressionDepositorInsert2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shStk16-1
Plasmid#180391PurposeProducing AAV that encodes mouse Stk16 shRNA-1 with miR-E backboneDepositorInsertshStk16-1 (Stk16 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMB37
Plasmid#73084PurposeExpresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp16
Plasmid#157723Purposemammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoterDepositorInsertNsp16 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162336PurposeLentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterhU6; mU6Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
UseTagsExpressionInsectMutationPromoterhsp70Available sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Y771F EphA2 pcDNA3
Plasmid#102735PurposeExpression of EphA2 in mammalian cellsDepositorInsertEphA2 (Epha2 Mouse)
UseTagsExpressionMammalianMutationY771F (TAC > TTC)PromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Y593E EphA2 pcDNA3
Plasmid#102731PurposeExpression of EphA2 in mammalian cellsDepositorInsertEphA2 (Epha2 Mouse)
UseTagsExpressionMammalianMutationY593E (TAC > GAG)PromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp10
Plasmid#157706Purposemammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorInsertNsp10 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp1-HF
Plasmid#157681Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoterDepositorInsertHF-Nsp1 (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCI-Neo-gp78 R2M / JM21
Plasmid#13304DepositorInsertgp78 (AMFR Human)
UseTagsExpressionMammalianMutationC356G ; H362APromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
shYES1 # 2
Plasmid#42547DepositorInsertYES1 (YES1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S285A/T289A-3xFLAG
Plasmid#69841PurposeThis plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS285A/T289APromoterCMVAvailable sinceDec. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-PLK4-S305E-3xFLAG
Plasmid#69840PurposeThis plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tagDepositorInsertPLK4 (PLK4 Human)
UseTags3xFLAG tag and EGFPExpressionMammalianMutationS305EPromoterCMVAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-PKA-RIIβ-sREACh
Plasmid#114113PurposeMammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.DepositorInsertPKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-hINO80N2 (1-406)
Plasmid#29442DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 1-406 (a.a.)PromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon3_2_Lb
Plasmid#155054PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40-hCas9 L3-L2
Plasmid#62133PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloningDepositorInserthuman codon optimized Cas9
UseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only