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Plasmid#72018PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.f-AP-His
Plasmid#71968PurposeExpresses the extracellular region of the Neogenin 1, isoform f protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.f (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.g-AP-His
Plasmid#71969PurposeExpresses the extracellular region of the Neogenin 1, isoform g protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.g (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Neo1.d-AP-His
Plasmid#71966PurposeExpresses the extracellular region of the Neogenin 1, isoform d protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.d (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_AB
Plasmid#66016PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_EB
Plasmid#66017PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_FB
Plasmid#66018PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_GB
Plasmid#66019PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0033m_BC
Plasmid#66021PurposeMoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
B0034m_BC
Plasmid#66022PurposeMoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD12_BC
Plasmid#66023PurposeMoClo Basic Part: RBS - BiCistronic Design, medium strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD12:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD2_BC
Plasmid#66024PurposeMoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCD8_BC
Plasmid#66025PurposeMoClo Basic Part: RBS - BiCistronic Design, low strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD8:C]DepositorInsertRBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0040 (tetR)_CD
Plasmid#66027PurposeMoClo Basic Part: CDS - Controller protein, tetR repressor (represses pTet, C0040. can be inhibited by tetracyclin or aTc) [C:C0040:D]DepositorInsertTranscription factor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0080 (araC)_CD
Plasmid#66029PurposeMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]DepositorInsertTranscription factor
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_EB
Plasmid#66005PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_FB
Plasmid#66006PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_GB
Plasmid#66007PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
UseTagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-R5A-K6A C11orf83-V5
Plasmid#65850PurposeMammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6ADepositorInsertMutated UQCC3 (UQCC3 Human)
UseTagsV5ExpressionMammalianMutationR5A-K6APromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only