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Plasmid#106301PurposeExpresses SHMT2DepositorInsertserine hydroxymethyltransferase 2 (SHMT2 Human)
UseRetroviralTagsExpressionMammalianMutationSilent mutations destroy sgRNA targeting sitesPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB73
Plasmid#73086PurposeExpresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75232PurposeCRISPR/Cas9 plasmid against human NFATc2DepositorInsertsgRNA against NFATc2 (NFATC2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-d
Plasmid#136295Purposeexpresses ABE8.8-d in mammalian cellsDepositorInsertABE8.8-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describeā¦PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp1
Plasmid#157680Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp1 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CamKII-ArchT-GFP (PV2527)
Plasmid#99039PurposeAAV-mediated expression of ArchT under the CamKII promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertArchT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterCamKIIAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB117 - pL2_pSB90_fLUC-I_V2
Plasmid#123192Purposebinary plant vector for transient TYLCV V2 and fLUC (with intron) expressionDepositorInsert2x35S::V2::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-UPP1_sgRNA2
Plasmid#201635PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertUPP1 (UPP1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13
Plasmid#157712Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWay21-MKP-1 ASA
Plasmid#13479DepositorInsertMKP-1ASA (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Arginine 53 to Alanine, Changed Arginineā¦PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-FRT-TO-Nsp1
Plasmid#157679Purposemammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoterDepositorInsertNsp1 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nsp5
Plasmid#157685Purposemammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoterDepositorInsertNsp5 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-Nprot
Plasmid#157728Purposemammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertNprot (N SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB71
Plasmid#73085PurposeExpresses C. elegans optimized BirA under control of the intestinal elt-2 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64T acvr1ba*
Plasmid#185409PurposeConstitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)DepositorInsertacvr1ba (acvr1ba Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Threonine 206 to Aspartic acidPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_IL1RN
Plasmid#64151PurposePhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA3_IL1RN (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
hRAD18 dSAP-EGFP
Plasmid#68825PurposeExpresses human RAD18 (deleting SAP domain) tagged with EGFPDepositorInserthuman RAD18 (deleting SAP domain) tagged with EGFP (RAD18 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp9-HF
Plasmid#157705Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoterDepositorInsertNsp9-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pORANGE CACNG8-GFP KI #2
Plasmid#131474PurposeEndogenous tagging of TarpĪ³8: Intramolecular (amino acid position: A408)DepositorInsertgRNA and GFP donor (Cacng8 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only