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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3

Plasmid

#109024

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA-DsRed-Express2

Plasmid

#111257

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Expression

Bacterial

Promoter

PpsbA

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Flag-Ube2v1

Plasmid

#110069

Purpose

Express Halo-Tev-Flag-Ube2v1 (human) in mammalian cells

Depositor

Yimon Aye

Article

Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

Insert

Halo-Tev-Flag-Ube2v1 (UBE2V1 Human)

Tags

Halo-TEV-Flag

Expression

Mammalian

Promoter

T7

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBXNPH3

Plasmid

#110098

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tet1 CD Mut

Plasmid

#72218

Purpose

Express flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.

Depositor

Yi Zhang

Article

Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

Insert

Ten-Eleven Translocation 1 (Tet1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

The plasmid expresses TET1 catalytic domain (cata…

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
CBh-dCas9-Mll3SET

Plasmid

#108452

Purpose

Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

Depositor

Bing Ren

Article

Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

Insert

dCas9, MLL3 (Kmt2c Mouse)

Expression

Mammalian

Promoter

CBh

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon1-KGC-EGFP-ER2

Plasmid

#108429

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon1-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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SAC1deltaTMD-FKBP-mCherry

Plasmid

#108123

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ150-Cas9(dpiRNA)

Plasmid

#107940

Purpose

eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

Depositor

Heng-Chi Lee

Article

Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

Insert

Cas9(dpiRNA)

Expression

Worm

Promoter

eft-3

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

Plasmid

#106177

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4_ELAVL1

Plasmid

#106103

Purpose

Entry vector for ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Use

Gateway Cloning

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING R232

Plasmid

#102608

Purpose

Retroviral vector to expression STING R232

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R232

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/Ecad-tdTomato

Plasmid

#101278

Purpose

Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

tdtomato

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCFP-LacI-NLS

Plasmid

#103840

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CFP-LacI-NLS

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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