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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Publication

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
hBACCS2-IRES-mCherry

Plasmid

#72892

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

Depositor

Takao Nakata

Publication

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hBACCS2-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST53-LRRK2-3XKD-R1441C

Plasmid

#25050

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
GAL FLAG72QDProCFPp303

Plasmid

#15586

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-SpHis5

Plasmid

#44846

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKeima-CaURA3

Plasmid

#44885

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomWasabi-Kan

Plasmid

#44945

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomApple-Kan

Plasmid

#44957

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomApple

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

Plasmid

#220727

Purpose

AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13563 - pAAV-AiE0170h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3563)

Plasmid

#224029

Purpose

AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214506

Purpose

AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CG

Plasmid

#116774

Purpose

Lentiviral expression of PIK3CG

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CG (PIK3CG Human)

Use

Lentiviral

Mutation

WT

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-WDR12

Plasmid

#116803

Purpose

Lentiviral expression of WDR12

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

WDR12 (WDR12 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-SMAD4

Plasmid

#116791

Purpose

Lentiviral expression of SMAD4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMAD4 (SMAD4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP3K4

Plasmid

#116759

Purpose

Lentiviral expression of MAP3K4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP3K4 (MAP3K4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT4

Plasmid

#116745

Purpose

Lentiviral expression of FLT4

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-BMPR2

Plasmid

#116718

Purpose

Lentiviral expression of BMPR2

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BMPR2 (BMPR2 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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