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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Publication

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-HA-CCND1

Plasmid

#172649

Purpose

Expresses HA-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCND1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK4

Plasmid

#172612

Purpose

Expresses HA-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDK4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 WT

Plasmid

#171485

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

Depositor

Veerle Baekelandt

Publication

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 (ATP13A2 Human)

Use

Lentiviral

Mutation

D508N , D962N

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB418

Plasmid

#74812

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB420

Plasmid

#74814

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEvol-NnSULT1C1-cysDNCQ

Plasmid

#188983

Purpose

biosynthesize sulfotyrosine in E. coli.

Depositor

Han Xiao

Publication

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Inserts

NnSULT1C1
cysD
cysN
cysC
cysQ

Expression

Bacterial

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Publication

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Promoter

CMVp

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-TagGFP2

Plasmid

#121426

Purpose

TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Publication

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

empty TagGFP2

Use

Lentiviral

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Publication

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Tags

GST

Expression

Bacterial

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Publication

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Publication

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
hBACCS2-IRES-mCherry

Plasmid

#72892

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

Depositor

Takao Nakata

Publication

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hBACCS2-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST53-LRRK2-3XKD-R1441C

Plasmid

#25050

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
GAL FLAG72QDProCFPp303

Plasmid

#15586

Depositor

Susan Lindquist

Publication

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP657-SpHis5

Plasmid

#44846

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP657

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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