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pAAV-DIO-TVA-V5-RG

Plasmid

#119743

Purpose

Expresses TVA, V5, and RG in a cre dependent manner.

Depositor

Marie Carlén

Article

Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

Insert

TVA, V5, Rabies Glycoprotein

Use

AAV and Cre/Lox

Tags

V5

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

Plasmid

#122202

Purpose

Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

Depositor

Raphael Gaudin

Article

Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

Inserts

HBc from HBV AYW
mNeonGreen

Tags

GGSGGSGGS linker

Expression

Mammalian

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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KA1153_pPBCAG-MerCreMer-IN

Plasmid

#124183

Purpose

piggyBac-based expression of the tamoxifen-inducible Cre

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

MerCreMer-IRES-Neo

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA0637_pPBCAG-rtTAM2-IN

Plasmid

#124166

Purpose

piggyBac-based expression of rtTA-M2

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

rtTAM2-IRES-Neo

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306-PHIS3-GFP-μNS

Plasmid

#116935

Purpose

Yeast integrative vector for the expression of μNS

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

μNS

Tags

GFP

Expression

Yeast

Promoter

HIS3

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMTMVi-N

Plasmid

#118756

Purpose

Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus cDNA fragment with a cloning polylinker

Use

Intermediate to build tobacco mosaic virus recomb…

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-SV40

Plasmid

#117811

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-SV40

Plasmid

#117815

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-TagRFP-SV40

Plasmid

#117805

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSH231-Bx-GFP-C31

Plasmid

#115149

Purpose

Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Safe Harbor Homology Sequences

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1

Plasmid

#119855

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-blueCKAR

Plasmid

#118472

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-blueAKAR

Plasmid

#118470

Purpose

cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

blueAKAR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pZ:F3-CAGGS GPHTK-F

Plasmid

#112666

Purpose

Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

Gene targeting vector for integration at the s1 s…

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Article

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3

Plasmid

#109024

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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