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pIDK-HDAC1fl

Plasmid

#109158

Purpose

Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Publication

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)

Expression

Insect

Promoter

p10

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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SB_concave

Plasmid

#110102

Purpose

pBXPHM3 with scaffold Sybody of the concave library

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the concave library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti-HA-mBec1 F121A

Plasmid

#99506

Purpose

Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

Depositor

Congcong He

Publication

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Mutation

F121A

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIRESpuro2-UBXD1-mCherry

Plasmid

#31835

Depositor

Hemmo Meyer

Publication

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UBXD1 (UBXN6 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Publication

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Publication

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

P200H

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Publication

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

I179N

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

Plasmid

#225828

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Publication

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

mVenus-FKBP-K7GEF

Use

AAV

Expression

Mammalian

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

Plasmid

#165450

Purpose

Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

Depositor

Elvir Becirovic

Publication

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9N-IntN
3x Opn1mw promoter-targeting sgRNAs

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6 and short human rhodopsin promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-HA-CCND1

Plasmid

#172649

Purpose

Expresses HA-tagged cyclin D1 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCND1 (CCND1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK4

Plasmid

#172612

Purpose

Expresses HA-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDK4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti HsATP13A2 WT

Plasmid

#171485

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

Depositor

Veerle Baekelandt

Publication

van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

Insert

human ATP13A2 (ATP13A2 Human)

Use

Lentiviral

Mutation

D508N , D962N

Promoter

CMV

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Publication

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB418

Plasmid

#74812

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGWB420

Plasmid

#74814

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEvol-NnSULT1C1-cysDNCQ

Plasmid

#188983

Purpose

biosynthesize sulfotyrosine in E. coli.

Depositor

Han Xiao

Publication

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Inserts

NnSULT1C1
cysD
cysN
cysC
cysQ

Expression

Bacterial

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-TagGFP2

Plasmid

#121426

Purpose

TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Publication

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

empty TagGFP2

Use

Lentiviral

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Publication

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Tags

GST

Expression

Bacterial

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p-EGFP-C1-Flag-mFmr1(D)

Plasmid

#87914

Purpose

Expresses mouse FMRP-EGFP fusion with phosphorylation mimic S499D.

Depositor

Stephanie Ceman

Publication

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

S499D

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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