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Plasmid#206284PurposeBacterial expression of Cas9-His Δcys E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E945CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL24_Cas9-His Δcys E1026C
Plasmid#206285PurposeBacterial expression of Cas9-His Δcys E1026C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1026CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL27_Cas9-His Δcys E532C/E945C
Plasmid#206288PurposeBacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574S, E945CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL26_Cas9-His Δcys M1C/E532C
Plasmid#206287PurposeBacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL22_Cas9-His Δcys E532C
Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorInsertVEGFA (Vegfa Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB91
Plasmid#199319PurposeCombine with Gal4 to direct ACR1 expressionDepositorInsert15xUAS::delta pes-10::::ACR1::let-858 3'UTR
UseTagsExpressionWormMutationPromoter15xUAS::delta pes-10Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG165
Plasmid#202584PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG161
Plasmid#202580PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG160
Plasmid#202579PurposeRNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG159
Plasmid#202578PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-barDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Talin-T100-L432G-Cterminal-mEmerald
Plasmid#202371PurposeTalin(FullLength)-L432G-mEmeraldDepositorInsertTalin(FullLength)-L432G-mEmerald
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
UseTagsmCherry (K187N -please see depositor comments)ExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP
Plasmid#188274PurposeMammalian expression, Genome editingDepositorInsertPlmCasX(T26R/K610R/K808R)
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF2
Plasmid#200376PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJPT020-LAT_dCore-HaloTag-3x FLAG
Plasmid#202504PurposeExpress dCore Lat mutantDepositorInsertLAT (LAT Human)
UseTagsHaloTag-3x FLAGExpressionMammalianMutationTruncated transmembrane domainPromoterCMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only