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Showing: 11721 - 11740 of 22020 results

  1. pLenti-ePEG-LINE1-loxPsym

    Plasmid
    #216866
    Purpose
    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements
    Depositor
    Leopold Parts
    Insert
    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216866-plasmid-map-sequence-id-441211

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  2. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  5. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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  6. pMS051

    Plasmid
    #137025
    Purpose
    SapTrap mScarlet-I-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mScarlet
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137025-plasmid-map-sequence-id-274042

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  7. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  8. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    None
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

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  9. ipUSEPR-sg-Hs-PHGDH-1

    Plasmid
    #188675
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188675-plasmid-map-sequence-id-375079

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  10. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  11. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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  12. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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  13. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

    Plasmid
    #221536
    Purpose
    EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs
    Depositor
    Omar Akbari
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221536-plasmid-map-sequence-id-437446

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  14. lentiGuideSLC35C1-neo

    Plasmid
    #220868
    Purpose
    encodes CRISPR sgRNA targeting human SLC3C1
    Depositor
    Jennifer Kohler
    Insert
    SLC35C1 (SLC35C1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220868-plasmid-map-sequence-id-440867

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  15. pGS96_EGFP(A206K)-I3-01 AAVS donor

    Plasmid
    #211865
    Purpose
    I3-01 transgene integration at AAVS1 safe harbor locus
    Depositor
    Iain Cheeseman
    Insert
    I3-01
    Use
    CRISPR
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211865-plasmid-map-sequence-id-426339

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  16. pR6K-crRNA-CASTIF

    Plasmid
    #199654
    Purpose
    R6K plasmid with a I-F CAST targeting lacZ
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199654-plasmid-map-sequence-id-412255

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  17. pR6K-crRNA-CASTIB

    Plasmid
    #199656
    Purpose
    R6K plasmid with a I-B CAST targeting lacZ
    Depositor
    IIya Finkelstein
    Insert
    CAST I-B systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199656-plasmid-map-sequence-id-412263

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  18. SGEP-sh-Hs-PHGDH-67

    Plasmid
    #188671
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188671-plasmid-map-sequence-id-375112

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  19. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188673-plasmid-map-sequence-id-375111

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  20. SGEP-sh-Hs-PHGDH-1957

    Plasmid
    #188674
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188674-plasmid-map-sequence-id-375081

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Showing: 11721 - 11740 of 22020 results