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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS L4137P-GST

Plasmid

#83455

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, L4137P

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO h-PKD1 FLS R4150C-GST

Plasmid

#83454

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, R4150C

Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pJK516

Plasmid

#71578

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

changes glutamate-435 to glutamine

Promoter

T7

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJK524

Plasmid

#71580

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

changes asparagine-347 to alanine

Promoter

T7

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJK513

Plasmid

#71576

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

Depositor

T. J. Kappock

Article

Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

changes glutamate-294 to alanine

Promoter

T7

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sdk2-Fc-His

Plasmid

#72135

Purpose

Expresses the extracellular region of the Sdk2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sdk2 (Sdk2 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3g.a(S)-Fc-His

Plasmid

#72152

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3g.z(S)-Fc-His

Plasmid

#72153

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Unc5a.a-AP-His

Plasmid

#72050

Purpose

Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a.a (Unc5a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Rgma-Fc-His

Plasmid

#72133

Purpose

Expresses the extracellular region of the RGMa protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Rgma (Rgma Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema4f.a-Fc-His

Plasmid

#72158

Purpose

Expresses the extracellular region of the Sema4F, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4f.a (Sema4f Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3g.z(S)-AP-His

Plasmid

#72027

Purpose

Expresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.z (Sema3g Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema6d.5-Fc-His

Plasmid

#72171

Purpose

Expresses the extracellular region of the Sema6D, isoform 5 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.5 (Sema6d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3d(S)-Fc-His

Plasmid

#72144

Purpose

Expresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema6a.z-AP-His

Plasmid

#72038

Purpose

Expresses the extracellular region of the Sema6A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.z (Sema6a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge