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Showing: 11701 - 11720 of 16226 results

  5. pCas1_Cas2/3

    Plasmid
    #89240
    Purpose
    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1
    Depositor
    Blake Wiedenheft
    Article
    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p
    Insert
    Cas1 and Cas2/3
    Use
    Tags
    Strep II tag on Cas1
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89240-plasmid-map-sequence-id-176381

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  6. pTK313

    Plasmid
    #59570
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP XbaI attB1 Kozak EGFP-PEST ochre attB2 AscI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59570-plasmid-map-sequence-id-92975

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  11. pAH237

    Plasmid
    #121146
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Mutation
    Promoter
    S.pombe rrk1 and nmt41
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121146-plasmid-map-sequence-id-234796

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  13. pFC331

    Plasmid
    #87843
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and argB selection marker
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus nidulans tef1 promoter and native pro…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87843-plasmid-map-sequence-id-177947

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  15. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    70019-plasmid-map-sequence-id-124558

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Showing: 11701 - 11720 of 16226 results