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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-Q61L

Plasmid

#12981

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 constitutively active (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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p5M-PR-A

Plasmid

#89124

Purpose

mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-H

Plasmid

#182281

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-H (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRPF215

Plasmid

#106377

Purpose

C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

Depositor

Neil Fairweather, Robert Fagan

Article

Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

Insert

Ptet-himar1-Ter(slpA)-transposon

Use

E. coli - c. difficile shuttle vector

Tags

Expression

Mutation

Codon optimised for C. difficile

Promoter

Ptet

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
HA-Vps34

Plasmid

#86749

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Vps34 (PIK3C3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Article

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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IVT-nCas9(H840A)

Plasmid

#207455

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

H840A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

Plasmid

#200406

Purpose

Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

MCP-ADAR2dd(E488Q)-NES

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

E488Q (hyperactive)

Promoter

CMV

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT194

Plasmid

#202293

Purpose

Type IIS cloning plasmid for LPattB5 integrations w/ OriT

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-Drp1-StrepII

Plasmid

#174422

Purpose

Expresses human Drp1 Isoform 3 in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (WT)

Plasmid

#24802

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge