Skip to main content
Addgene
Showing: 11701 - 11720 of 21160 results
  1. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid
    #114070
    Purpose
    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag
    Insert
    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR

    Plasmid
    #171382
    Purpose
    Expression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Insert
    humanized dCas13X.1-REPAIRv2
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Insert
    humanized minidCas13X.1-REPAIRv2
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 11701 - 11720 of 21160 results