Addgene

We narrowed to 24,268 results for: cat

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GFP-RAB10 K154A

Plasmid

#130886

Purpose

Expresses human RAB10 K154A mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

K154A

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-1899)

Plasmid

#170145

Purpose

Expresses residues 787-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-2138)

Plasmid

#170146

Purpose

Expresses residues 787-2138 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Use

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp5

Plasmid

#157686

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp5 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pORANGE Cplx2-GFP KI

Plasmid

#131476

Purpose

Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-Itprip-V5

Plasmid

#120241

Purpose

Expresses V5-tagged Itprip in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Itprip (ITPRIP Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMB72

Plasmid

#73088

Purpose

C-terminal GFP-2xTEV-Avi vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

GFP-Avi tag

Use

Tags

GFP and Avi

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Orf6

Plasmid

#157727

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Orf6 (ORF6 SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-FAM86A

Plasmid

#85117

Purpose

bacterial expression of FAM86A

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EEF2KMT (FAM86A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4254

Plasmid

#89050

Purpose

Expresses St chimeric gRNA and human codon-optimized StCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

St chimeric gRNA
hStCas9

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-tTA

Plasmid

#127091

Purpose

An AAV genome with Cre-dependent expression of tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ArchT-GFP

Plasmid

#63141

Purpose

AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

human synapsin promoter

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 K164A/H165A CoV2

Plasmid

#175514

Purpose

For protein expression of K164A/H165A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

K164A/H165A nsp1 CoV2

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX nsp1 R124A/K125A CoV2

Plasmid

#175513

Purpose

For protein expression of R124A/K125A nsp1 CoV2

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

R124A/K125A nsp1 CoV2

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX C_K nsp1 K164A/H165A CoV2

Plasmid

#175517

Purpose

Expresses C_K for bioconjugation on nsp1

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

C_K nsp1 CoV2

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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NPRL2_pLX307

Plasmid

#98364

Purpose

Lentiviral expression of NPRL2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

NPRL2 (NPRL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-WHaloCaMP1a-EGFP

Plasmid

#205309

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the CaMKII promoter

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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