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pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-TPNOX

Plasmid

#87853

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

TPNOX

Use

Tags

C-terminal FLAG tag with a linker

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDN-Sth1Cas9

Plasmid

#221386

Purpose

Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

Sth1 Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDN-Cherry-sgRNA

Plasmid

#221387

Purpose

episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

Depositor

Deborah Hung

Article

Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

Insert

none, cloning vector for introduction of targeting sequences

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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HIR K1030R

Plasmid

#24050

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

K1030R

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GFP-h-PKD2

Plasmid

#83451

Purpose

GFP-tagged PC2 for Eukaryotic expression (883)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-2 (PKD2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-Klf5

Plasmid

#66657

Purpose

For expression of mouse Klf5.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf5 (Klf5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pADV-Sec61b-TurboID

Plasmid

#179143

Purpose

Recombinant adenoviral plasmid for Sec61b-TurboID expression

Depositor

Jae Myoung Suh

Article

Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

Insert

Sec61b-V5-TurboID

Use

Adenoviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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TPC2-G-GECO1.2

Plasmid

#207141

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Use

Tags

TEV-3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EGFP

Plasmid

#89875

Purpose

EGFP reporter vector of iTango system for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5M-ADAM10

Plasmid

#31717

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

ADAM10 (ADAM10 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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HIR WT

Plasmid

#24049

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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FRESCA-syntide

Plasmid

#132415

Purpose

FRET-based sensor for CaMKII activity

Depositor

Margaret Stratton

Article

Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

Insert

FRESCA-syntide

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

CARM1 (Carm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3_2FLAG-AKAP79 WT

Plasmid

#183450

Purpose

Expression of human wild-type AKAP79

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE57

Plasmid

#90402

Purpose

Type I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GR

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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