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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp13

Plasmid

#157713

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp13 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

Plasmid

#127865

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

deleted the c-terminal 20 amino acids

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pPKm-105

Plasmid

#104853

Purpose

pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PhyB NT GBD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPN062

Plasmid

#91595

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p6608 MSCV-IP N-HAonly CHAF1B

Plasmid

#34901

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHAF1B (CHAF1B Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C3-PLK4-S305A-3xFLAG

Plasmid

#69839

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305A

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHA

Plasmid

#177980

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

Depositor

Jessica Spinelli, David Sabatini

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHA (SDHA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TS-PE2-C-WPRE

Plasmid

#176650

Purpose

Expresses C-terminus of PE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

C-terminus of PE2
Artificial SA
WPRE
bGH poly(A)

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

None

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMB41

Plasmid

#73087

Purpose

N-terminal Avi-2xTEV-GFP vector, for fosmid recombineering

Depositor

Mike Boxem

Article

Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

Insert

Avi-GFP tag

Use

Tags

Avi and GFP

Expression

Worm

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

Plasmid

#124660

Purpose

reporter protein which emits bright blue fluorescence

Depositor

Yutaka Kuroda

Article

Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

Insert

Gaussia luciferase-C9D-TGinsert

Use

Tags

IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

Expression

Bacterial

Mutation

changed glutamic acid 100 to alanine, and glycine…

Promoter

T7 promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

Plasmid

#117384

Purpose

An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Y921F EphA2 pcDNA3

Plasmid

#102736

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y921F (TAC > TTC)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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iBE3 CLYBL

Plasmid

#174569

Purpose

doxycycline inducible BE3 expression for human CLYBL locus knock-in

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

tet ON

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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OXSM_pLX307

Plasmid

#98357

Purpose

Lentiviral expression of OXSM

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

OXSM (OXSM Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp9

Plasmid

#157703

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp9 (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-cryR;dll4CE1-P1Egfp

Plasmid

#90146

Purpose

contains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E1P1Egfp (dll4 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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