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Plasmid#162760PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeTagsExpressionPlantMutationPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700 HL1gR4
Plasmid#124450PurposeExpresses gRNA HL1 gR4 in the pSB700 backboneDepositorInsertHL1 gR4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM65881
Plasmid#153215PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformationDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgTelo-MTSa/EGFP/pdCas9-C1
Plasmid#162759PurposeExpressing dCas9 and sgRNA containg MTSa targeting telomeresDepositorInsertdCas9 and sgRNA(SL2-sgTelo-MTSa)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ003 (LshC2c2 locus)
Plasmid#70168PurposeExpresses LshC2c2 full locusDepositorInsertLshC2c2 full locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D-peg
Plasmid#141081PurposeGateway compatible prime editing gRNA empty backbone vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55297
Plasmid#153213PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SL2-sgSat-MTSb/BFP/pdCas9-C1
Plasmid#162761PurposeExpressing dCas9 and sgRNA containg MTSa targeting centromeresDepositorInsertdCas9 and sgRNA(SL2-sgSat-MTSb)
UseTagsExpressionMammalianMutationdCas9(nuclease deactivated Cas9)PromoterU6/CMVAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
sg1+sg2+sg3
Plasmid#113969PurposeTriple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg1+sg2+sg3
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CCR5_gRNA_pX459
Plasmid#192268PurposegRNA expression for CRISPR Genome Editing of CCR5DepositorInsertCCR5 guide RNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS21: pHelper(PmcCAST)_entry
Plasmid#168154PurposeInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKMD106h - intein-Cas9(C574)
Plasmid#64191PurposeExpresses intein-Cas9(C574) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA3.8
Plasmid#121958PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA, short version
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only