-
Plasmid#112855PurposeDox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent proteinDepositorInsertDCHS1 (DCHS1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterdoxycycline inducible promoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-BSD-tet-mEGFP-MIB1
Plasmid#140241PurposeLentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatmentDepositorInsertTet-inducible mEGFP-MIB1 (MIB1 Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterTREAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-q-YFP
Plasmid#55782PurposeThis G protein alpha-q construct contains internal insertions of YFP and the EE epitope.DepositorInsertalpha-q EE YFP (Gnaq Mouse)
UseTagsThe EE epitope was introduced into the alpha-q se…ExpressionMammalianMutationBamHI and SacI sites in alpha-q were removed with…PromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPEN Beta2-microglobulin
Plasmid#215657PurposeE. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.DepositorInsertOPEN Beta2-microglobulin (B2M Human)
UseTagsExpressionBacterialMutationHistidine 31 to CysteinePromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF706
Plasmid#88037PurposeDonor Vector containing ZNF706 transcription factor, part of the Human TFome CollectionDepositorInsertZNF706 (ZNF706 Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MLL2 653
Plasmid#11017DepositorInsertMLL2 (KMT2B Human)
UseTagsFLAGExpressionMammalianMutationtruncation of MLL2, last 653 amino acids, from pr…PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26
Plasmid#92143PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D
Plasmid#169912PurposeExpression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.DepositorInsertdCjCas9
UseCRISPRTags10XGCN4, 2xSV40NLS, and SV40NLS-HAExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR TPP1 MBP TEV TIN2
Plasmid#185446PurposeCoexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF-FH-TAZ-ires-blast
Plasmid#52083PurposeLentiviral expression vector for TAZ with N-terminal FLAG and His tagsDepositorInsertTAZ (WWTR1 Human)
UseLentiviralTagsFLAG and HisExpressionMammalianMutationPromoterEF1aAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)
Plasmid#169188PurposeTo express His-SUMO-nsp12 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYUU
Plasmid#159751PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …TagsExpressionMutationPromotergfapAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HLA-A0201-His
Plasmid#108213PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseEntry vector for gateway systemTagshisExpressionMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL A WT
Plasmid#202413PurposeExpression of GFP-tagged PODXL (isoform A) WTDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
NC14 pGLUE FBXW11
Plasmid#36969DepositorInsertFBXW11 (FBXW11 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB3-SV40-GFP (B3G)
Plasmid#65443PurposeExpression of EPHB3 and GFP in mammalian cellsDepositorInsertEPHB3 (EPHB3 Human)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationPromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA
Plasmid#204547PurposeExpresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cellsDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsExpressionMammalianMutationdeltaUBAPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only