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pBT234_(pCA-G-intron-tTA2)

Plasmid

#36874

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPI TuDmiR122Gpluc7xlet7BT

Plasmid

#35648

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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TAL3072

Plasmid

#41246

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-fan1-Left (si:ch211-201b11.2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3073

Plasmid

#41247

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-fan1-Right (si:ch211-201b11.2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pAAVscCBPITuDlet-7Gpluc7x122BT

Plasmid

#35650

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD let-7

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pACYC-yhaM

Plasmid

#207650

Purpose

Overexpression of native L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaM

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5c-cdl

Plasmid

#207649

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-cdl

Plasmid

#207652

Purpose

Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

cdl

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-decR

Plasmid

#207653

Purpose

Overexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

decR

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMF2-yhaO

Plasmid

#207654

Purpose

Overexpression of L-cysteine importer in E. coli MG1655

Depositor

Benjamin Woolston

Article

Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

Insert

yhaO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBp

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8823

Plasmid

#220901

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8835

Plasmid

#220902

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8921

Plasmid

#220903

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP8923

Plasmid

#220904

Purpose

MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novo

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8930

Plasmid

#220905

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP8932

Plasmid

#220906

Purpose

MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

Depositor

Jason Peters

Article

Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

Insert

dCas9-novo

Use

CRISPR

Tags

3xMyc

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT011

Plasmid

#225162

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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3XHA-PP2A-Ba

Plasmid

#195229

Purpose

Retroviral vector containing HA tagged PP2A

Depositor

Keith Mostov, David Bryant

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PPP2R2A (PPP2R2A Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT008

Plasmid

#225159

Purpose

Low copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

FLAG epitope tag (35 bp)

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge