-
Plasmid#115367PurposeExpression vector to produce humanized Renilla luciferase with a single perfectly complementary binding site for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet-MBP
Plasmid#209184PurposeExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coliDepositorTypeEmpty backboneUseTagsHisX6ExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorInsertLactadherin-C2 (MFGE8 Bovine)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorInsertTIA1 (TIA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
190_SMAD4 in pmiRGlo
Plasmid#78128Purposeluciferase reporter for miRNA activityDepositorInsertSMAD4 (SMAD4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z22
Plasmid#120065PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD-IRP
Plasmid#220751PurposePlasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.DepositorInsertIron Regulatory Protein (ACO1 )
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcDNA3
Plasmid#18964DepositorInsertFirefly Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-EGFP
Plasmid#52343PurposeAAVS1 donor vector for inducible gene expressionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Tet1CD
Plasmid#84475PurposeLentiviral construct to express dCas9 fused with the catalytic domain of Tet1DepositorInsertdCas9-Tet1CD
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP
Plasmid#137763PurposeControl plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.DepositorInsertGFP
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-CAG-hrGFP
Plasmid#52344PurposeAAVS1 donor vector for constitutive gene expressionDepositorInserthrGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB-GLUT4-7myc-GFP
Plasmid#52872Purposedetection and measurement of GLUT4 translocationDepositorInsertGLUT4 (Slc2a4 with human c-terminus, Rat)
UseRetroviralTags7x-myc (internal) and GFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HRE-luciferase
Plasmid#26731PurposeLuciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.DepositorInsertHypoxia Responsive Elements (3)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only