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pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

Plasmid

#32460

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

S129D, S133D, S137D

Promoter

human PGK

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-CaMKKbeta S129A, S133A, S137A

Plasmid

#32454

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129A, S133A, S137A

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat S100A, S495A and S511A

Plasmid

#33320

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S99A, S494A, S510A

Promoter

SV40

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300A

Plasmid

#62526

Purpose

Serine 300 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302A

Plasmid

#62527

Purpose

Serine 302 of Drosha is mutated to alanine

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to alanine (DROSHA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to alanine

Promoter

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry NEDD4 WW2

Plasmid

#111882

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4 WW2 fused to C-termin…

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(1-280)

Plasmid

#78752

Purpose

To overexpress MKRN1(1-280) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-280

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(110-482)

Plasmid

#78754

Purpose

To overexpress MKRN1(110-482) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

110-482

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-B2-ELP-HIS-ybbR

Plasmid

#169134

Purpose

E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Use

Tags

3xELP linker, 6x Histag, and ybbr tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-FIVAR-ELP-HIS-ybbR

Plasmid

#169136

Purpose

E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

FIVAR (found in various architectures)

Use

Tags

3xELP linker, 6x Histag, Fgβ peptide, and ybbr ta…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-B1-ELP-HIS-ybbR

Plasmid

#169133

Purpose

E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Use

Tags

3xELP linker, 6x Histag, and ybbr tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Methylosarcina_fibrata

Plasmid

#224406

Purpose

For expression of the type I CBASS system encoded by Methylosarcina fibrata

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

4TM_MfCdnH_AGS-C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_vulnificus

Plasmid

#224405

Purpose

For expression of the type I CBASS system encoded by Vibrio vulnificus

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_Phospholipase_VvCdnG_AGS-C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

Plasmid

#224403

Purpose

For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Pseudoalteromonas_sp._A2

Plasmid

#224401

Purpose

For expression of the type I CBASS system encoded by Pseudoalteromonas sp. A2

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Psp.CdnB_AGS-C_Cap15

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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