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pcDNA6-rbNHERF1

Plasmid

#32942

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLC9A3R1 (SLC9A3R1 rabbit)

Use

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-APC11

Plasmid

#19900

Depositor

Yue Xiong

Article

Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

Insert

APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 N-MLSN

Plasmid

#21082

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM1 (TRPM1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 Glt1a

Plasmid

#98705

Purpose

mammalian expression of Glt1a

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

I288V (please see depositor comments below)

Promoter

CMV

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Synthetic, Human)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFPZNF598-9P-9A

Plasmid

#141192

Purpose

Expresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

ZNF598 (ZNF598 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

all 3 proline repeats are mutated to Alanine (9xP…

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B-V5

Plasmid

#35931

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D3

Plasmid

#155216

Purpose

Expresses human Muc2 residues 858-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D3 (MUC2 Human)

Use

Tags

6xHistadine

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Use

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNAFlagMenin(S543A)

Plasmid

#32083

Depositor

Matthew Meyerson

Article

Francis et al PLoS One. 2011 . 6(1):e16119.

Insert

Menin isoform 2 (MIR302B Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S543A

Promoter

CMV

Available since

Feb. 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-hBnvy

Plasmid

#18969

Depositor

Mariano Viapiano

Article

Hu et al J Biol Chem. 2008 Jul 7. ():.

Insert

Brevican uncleavable (BCAN Human)

Use

Tags

Expression

Mammalian

Mutation

Mutation at the ADAMTS cleavage site ESE/SRG: S…

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1MycHis/HIP1/ΔT

Plasmid

#31251

Depositor

Theodora Ross

Article

Rao et al J Clin Invest. 2002 Aug . 110(3):351-60.

Insert

Huntingtin-interacting protein 1 (HIP1 Human)

Use

Tags

his and myc

Expression

Bacterial

Mutation

deletion of TALIN region

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B

Plasmid

#120888

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7B-V5

Plasmid

#35934

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IP3KC

Plasmid

#32527

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

Insert

IP3KC (ITPKC Human)

Use

Tags

6xHIS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
YeNL-Actin_pcDNA3

Plasmid

#89521

Purpose

Yellow color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xt-VSP

Plasmid

#51887

Purpose

expression of Xt-VSP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

see comments*

Promoter

CMV

Available since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Glt1b

Plasmid

#98706

Purpose

mammalian expression of Glt1b

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

I348V and N365Y (please see depositor comments se…

Promoter

CMV

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge