Addgene

We narrowed to 1,538 results for: nif

Showing: 1141 - 1160 of 1538 results

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pDONR-IAA31

Plasmid

#83864

Purpose

Gateway donor vector for IAA31

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA31 (IAA31 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA16

Plasmid

#83854

Purpose

Gateway donor vector for IAA16

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA16 (IAA16 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

Plasmid

#39306

Depositor

Jennifer Tirnauer, Tim Mitchison

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

E211A, K212A, E213A, R214A, D215A and Y217A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
c-Fos

Plasmid

#187905

Purpose

Expresses c-Fos in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

cFos (FOS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1187 VSVGmut

Plasmid

#201913

Purpose

Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

VSV-G (K47Q, R354A)

Use

Tags

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Use

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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His-CL7-2NLS-SpyCas9(dS)-A22p3star

Plasmid

#222970

Purpose

Bacterial expression of A22p-fused SpyCas9 construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His-CL7-2NLS-SpyCas9(dS)-A22p3

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a

Plasmid

#113431

Purpose

Expresses 10xHis-MBP fused to LbCas12a

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Has Service

Cloning Grade DNA

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-GPI-RUSH

Plasmid

#166903

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-Halo-GPI

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Plasmid

#222992

Purpose

Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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Sar1b-YFP

Plasmid

#166897

Purpose

mammalian expression of Sar1b tagged with YFP

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-TfR-RUSH

Plasmid

#166906

Purpose

RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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JAB3603

Plasmid

#190422

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_4XPhlFO::GFPint-ADHt

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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