We narrowed to 5,041 results for: golden gate

Showing: 1141 - 1160 of 5041 results

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pCFJ2215 - Intron GG3 - 900 bp

Plasmid

#159882

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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BCJJ345

Plasmid

#117504

Purpose

YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtYAO

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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PBgg-Hygro

Plasmid

#248968

Purpose

Golden Gate assembly-friendly PiggyBac backbone with Hygromycin resistance

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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PBgg-Puro

Plasmid

#248967

Purpose

Golden Gate assembly-friendly PiggyBac backbone with Puromycin resistance

Depositor

James Briscoe

Article

Benzinger et al Dev Cell. 2025 Aug 19:S1534-5807(25)0049

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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GGDest2-Amp

Plasmid

#157650

Purpose

Golden Gate assembly of a pET28-compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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GGDest3-Amp

Plasmid

#157651

Purpose

Golden Gate assembly of a pTXB1 compatible fusion-protein ORF insert

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

none

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMC-Dest-BsaI

Plasmid

#200550

Purpose

Mini circle destination vector with BsaI sites for Golden Gate Assembly

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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L0-I35 p35S-XVE-pEstradiol GG0-GG1

Plasmid

#195904

Purpose

Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-XVE-LexA-p35S

Use

Synthetic Biology

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSB90 - pAGM4723_VirGN54D

Plasmid

#123187

Purpose

Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Type

Empty backbone

Use

Level 2 acceptor vector for expression in plants

Expression

Plant

Mutation

VirGN54D for increase virulence

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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PB_gRNA_S.pyogenes_scaffold_BB

Plasmid

#226429

Purpose

PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

Depositor

Alon Goren

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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L0-I39 PhiC31-Intein GG1-GG4

Plasmid

#195905

Purpose

Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

PhiC31-intein

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I18 Ub-Arg GG1-GG2

Plasmid

#195899

Purpose

Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Ub-Arg tag

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L0-I40 Intein-PhiC31 GG1-GG4

Plasmid

#195906

Purpose

Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Intein-PhiC31

Use

Synthetic Biology

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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BCJJ223

Plasmid

#117509

Purpose

AtMGE1 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pMGE1:AATG:BsaI

Expression

Plant

Promoter

AtMGE1

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL12021

Plasmid

#117511

Purpose

AtICU2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pICU2:AATG:BsaI

Expression

Plant

Promoter

AtICU2

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ433

Plasmid

#117507

Purpose

AtEC1.2 promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC1.2:AATG:BsaI

Expression

Plant

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSC9

Plasmid

#50429

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 9.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Available Since

Jan. 29, 2014

Availability

Academic Institutions and Nonprofits only

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EC71173

Plasmid

#154061

Purpose

Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Cre recombinase

Expression

Bacterial

Mutation

Introduction of U5 intron sequences, to prevent p…

Promoter

HvHSP17

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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PB-T3G-ERP2

Plasmid

#175188

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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