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Plasmid#165046PurposeIntegrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiaeDepositorInsertP-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1364-mouse U6-sgH2B
Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorInsertH2B targeting sgRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAP635-2
Plasmid#99485PurposeMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
S7_SwaI_Zeo_PacI
Plasmid#128471PurposeZeo Resistance geneDepositorInsertZeocin Resistance gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
S12_EcoRV_PGK_AscI
Plasmid#128476PurposePGK promoterDepositorInsertPGK promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDDIT3-donor
Plasmid#112300PurposeCRISPR donor plasmid to tag human transcription factor DDIT3 with GFPDepositorInsertDDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAID8Z104
Plasmid#165056PurposeIntegrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
UseTagsExpressionYeastMutationWTPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML1
Plasmid#108332PurposeMIDAS Level-1 source vector for cloning of modules (synthetic biology parts)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHOXB5-donor
Plasmid#113815PurposeCRISPR donor plasmid to tag human transcription factor HOXB5 with GFPDepositorInsertHOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
UseCRISPRTagsExpressionMutationrs2288276, rs7406798, rs36049616, rs3087996PromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike2
Plasmid#136949PurposePlasmid for the In Vitro transcription of DiySpike2DepositorInsertDiySpike2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NEO-CAG-BFP-pA-GFP-pA
Plasmid#149344PurposeVector for targeting the Replace reporter to the AAVS1 locusDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
B3rec.zf1
Plasmid#108354PurposeZebrafish codon optimized B3 recombinase that acts on blown-out sitesDepositorInsertB3 recombinase
UseSynthetic BiologyTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AP625-1
Plasmid#70051PurposeExpresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteriaDepositorInserteGFP
UseTags3Xflag::TEV::myc and myc::TEV::3XflagExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAP639-2F
Plasmid#99486Purpose3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlagDepositorInsert3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMST665_BB3_L 23_gRNAempty_cas9_BbsI
Plasmid#90278PurposeBB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9DepositorInsertCas9
UseA. nigerTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NLS KOZ c2
Plasmid#54443DepositorInsertNuclear localization signal (NLS) of SV40 large T antigen
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGD007
Plasmid#48837PurposeProvides linker:nuclear localization signal as GreenGate C-terminal tag module.DepositorInsertlinker:nuclear localization signal
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLOCK-donor
Plasmid#112310PurposeCRISPR donor plasmid to tag human transcription factor CLOCK with GFPDepositorInsertCLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)
UseCRISPRTagsExpressionMutationhg38:4:55435551:A>G, hg38:4:55435749:G>APromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP608-1
Plasmid#99484PurposeH2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTRDepositorInsertH2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP607-2
Plasmid#99483PurposeMyc::TEV::3XFlag::GFP::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::GFP::3XFlag::TEV::Myc
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
LI C-D 1-10GFP noATG
Plasmid#54462DepositorInsertsuperfolder GFP 1-10 domain without start codon
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pML3
Plasmid#108341PurposeMIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK571
Plasmid#75379PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)DepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
formylglycinamidine ribonucleotide synthetase, glutaminase subunit
formylglycinamidine ribonucleotide synthetase, large subunit
UseGene synthesisTagsHis6ExpressionBacterialMutationchanges glycine-209 to aspartate and synthetic ge…Promotern/aAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGRE NPD-GFP
Plasmid#1197DepositorInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
UseTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCas49
Plasmid#82391PurposerbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpKDepositorInsertrbcL-GFP
UseTagsExpressionMutationPromoterPccmK2Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
CJ41 (U1 snRNA (U1;21d))
Plasmid#17774DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
UseTagsExpressionMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
LI B-C N-NES KOZ b c7
Plasmid#54445DepositorInsertNuclear Export Signal (NES) of heat stable inhibitor (PKI)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pGGD_MUTT2A_E
Plasmid#218899Purposegolden gate cloningDepositorInsertMUTT2A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAN052
Plasmid#220046PurposeMammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tagsDepositorInsertFLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ221
Plasmid#212319PurposeExpress MmFnuc guide with pureexpressDepositorInsertMercenaria guide
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM032_P4_tSynth30
Plasmid#216457PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth30
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM031_P4_tSynth29
Plasmid#216456PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth29
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM030_P4_tSynth27
Plasmid#216455PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth27
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM029_P4_tSynth25
Plasmid#216454PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth25
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM028_P4_tSynth3
Plasmid#216453PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator synth3
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM027_P4_tSynthGuo
Plasmid#216452PurposeSynthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.DepositorInsertTerminator guo
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK-Spsg
Plasmid#214351PurposeExpresses cloning backbone for SpCas9 sgRNADepositorInsertSpCas9 tracrRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK-Sasg
Plasmid#214350PurposeExpresses cloning backbone for SlugCas9 sgRNADepositorInsertSlugCas9 tracrRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ238
Plasmid#212321PurposeExpress KnFnuc guide with pureexpressDepositorInsertKnFunc guide
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKJ217
Plasmid#212318PurposeExpress MmFnuc with pureexpressDepositorInsertMercenaria
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM752
Plasmid#207891PurposeLevel 0 for A5 module (5' ACCT-3' TCCC)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM761
Plasmid#207900PurposeLevel 0 for C7 module (5' GGCT-3' TTCG)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM759
Plasmid#207898PurposeLevel 0 for C5 module (5' AGCC-3' TCAG)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM760
Plasmid#207899PurposeLevel 0 for C6 module (5' TTCG-3' TCAG)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM764
Plasmid#207903PurposeLevel 0 for D3 module (5' TCCC-3' CTGC)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM767
Plasmid#207906PurposeLevel 0 for E1 module (5' CTGC-3' CCAT)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM751
Plasmid#207890PurposeLevel 0 for A4 module (5' TGAC-3' AACA)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM753
Plasmid#207892PurposeLevel 0 for B1 module (5' AACA-3' CCAT)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM762
Plasmid#207901PurposeLevel 0 for C8 module (5' GGCT-3' GCAG)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only