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Plasmid#188990PurposeA lentiviral plasmid encoding lysosome-tagged CIBNDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsp18ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_minCMV-sfGFP-CMV_dsRed Exp
Plasmid#117401PurposesfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoterDepositorInsertSuperFolder GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-GFP-mCherry-3pGW
Plasmid#53965PurposeBidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherryDepositorInsertsGFP
mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Blue opsin mcherry ---- pcDNA3.1
Plasmid#62932PurposeBlue opsin tagged with mcherry on the C terminusDepositorInsertblue opsin
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ-rfp
Plasmid#153037PurposeCRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-AtU3b-tRNA-ccdB-gRNA
Plasmid#118390PurposeEntry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertAtU3b promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
UseTagsExpressionBacterialMutationPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMH1105
Plasmid#131864PurposeProduces holo-PhyB-AviTag in E. coliDepositorInsertsPhyB(1-651)-AviTag-His6
HO1
PcyA
UseTagsExpressionBacterialMutationPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS415_pGal-nCas9(D10A)
Plasmid#79618PurposeExpresses nCas9(D10A) in yeast cellsDepositorInsertSpCas9
UseTagsExpressionYeastMutationD10A for nickase Cas9PromoterpGal1Available sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-smURFP-T2A-mCherry
Plasmid#80347PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.DepositorInsertssmURFP
mCherry
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
#1.4 post-R-SynapShot-Lyn-miRFP670
Plasmid#214718PurposeExpresses red #1.4 post-SynapShot and Lyn-miRFP670DepositorInsert#1.4 post-R-SynapShot
UseTagsLyn-miRFP670ExpressionMammalianMutationPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHLP710
Plasmid#203740PurposeIntegration of OsTIR1-3xMyc at NEUT5L locusDepositorInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycExpressionMutationPromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR pUAS-dGFP pPGK-iRFP
Plasmid#176530PurposeLentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.DepositorInsertsdGFP
iRFP
UseLentiviralTagsExpressionMammalianMutationPromoterPGKp and UASpAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
CENP-B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45214DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBT346_pLambda-attB-pCA-GFP-pA
Plasmid#52551Purposefor producing PhiC31 attB-containing minicircle in vitroDepositorInsertsLambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDE43-MCK
Plasmid#49523PurposeKanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-Logic-AND
Plasmid#112237PurposeGFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.DepositorInsertsGFP under control of doubly-repressible promoter
GFP
araC
UseTagsExpressionBacterialMutationPromoterAND-gate and pLacIAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
V2-MESA-35F-M-dCas9
Plasmid#84506PurposeMESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64DepositorInsertV2-MESA-35F-M-dCas9
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only